Evidence map›Paper›PMID 27110730›Full record

ReviewJournal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics2016

Nanofiber Scaffold-Based Tissue-Engineered Retinal Pigment Epithelium to Treat Degenerative Eye Diseases.

Nathan A Hotaling, Vladimir Khristov, Qin Wan, Ruchi Sharma, Balendu Shekhar Jha, Mostafa Lotfi, Arvydas Maminishkis, Carl G Simon, Kapil Bharti

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 69 citations in OpenAlex.

  1. Review
  2. Review
  3. Nanotherapy for Neural Retinal Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  4. Long-term cultivation of retinal pigment epithelium cells on nanofiber scaffolds.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2025
    Article
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  19. Nano-Biomaterials for Retinal Regeneration.Nanomaterials (Basel, Switzerland) · 2021
    Review
  20. Tissue Engineering Strategies for Retina Regeneration.Applied sciences (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nathan A Hotaling1 Biosystems and Biomaterials Division, National Institute of Standards and Technology , Gaithersburg, Maryland.
Vladimir Khristov3 Section of Epithelial and Retinal Physiology and Disease, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Qin Wan3 Section of Epithelial and Retinal Physiology and Disease, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Ruchi Sharma2 Unit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Balendu Shekhar Jha2 Unit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Mostafa Lotfi3 Section of Epithelial and Retinal Physiology and Disease, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Arvydas Maminishkis3 Section of Epithelial and Retinal Physiology and Disease, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
Carl G Simon1 Biosystems and Biomaterials Division, National Institute of Standards and Technology , Gaithersburg, Maryland.
Kapil Bharti2 Unit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health , Bethesda, Maryland.
National Eye Institute · USNational Institutes of Health · USNational Institute of Standards and Technology · US

Funding

Human Retinal Pigment Epithelial PhysiologyZIAEY000419 · NEI · NATIONAL EYE INSTITUTE · PI SCHNEEWEIS, DAVID · 2009 to 2020
$12.0M
6 · The paper itself

Abstract

Clinical-grade manufacturing of a functional retinal pigment epithelium (RPE) monolayer requires reproducing, as closely as possible, the natural environment in which RPE grows. In vitro, this can be achieved by a tissue engineering approach, in which the RPE is grown on a nanofibrous biological or synthetic scaffold. Recent research has shown that nanofiber scaffolds perform better for cell growth and transplantability compared with their membrane counterparts and that the success of the scaffold in promoting cell growth/function is not heavily material dependent. With these strides, the field has advanced enough to begin to consider implementation of one, or a combination, of the tissue engineering strategies discussed herein. In this study, we review the current state of tissue engineering research for in vitro culture of RPE/scaffolds and the parameters for optimal scaffold design that have been uncovered during this research. Next, we discuss production methods and manufacturers that are capable of producing the nanofiber scaffolds in such a way that would be biologically, regulatory, clinically, and commercially viable. Then, a discussion of how the scaffolds could be characterized, both morphologically and mechanically, to develop a testing process that is viable for regulatory screening is performed. Finally, an example of a tissue-engineered RPE/scaffold construct is given to provide the reader a framework for understanding how these pieces could fit together to develop a tissue-engineered RPE/scaffold construct that could pass regulatory scrutiny and can be commercially successful.

Indexed as

Tissue EngineeringAnimalsHumansNanofibersRetinal DegenerationRetinal Pigment EpitheliumTissue Scaffolds

Identifiers

PMID27110730
PMCPMC4904235
OpenAlexW2343918639

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.