Evidence map›Paper›PMID 27189940›Full record

ArticleThe Journal of biological chemistry2016

Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles.

Barbara Mosca, Jan Eckhardt, Leda Bergamelli, Susan Treves, Rossana Bongianino, Marco De Negri, Silvia G Priori, Feliciano Protasi, Francesco Zorzato

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. ERG1A KScientific reports · 2025
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Barbara MoscaDepartment of Life Science and Biotechnology, University of Ferrara, Via Borsari 46, 44100, Ferrara, Italy.
Jan EckhardtFrom the Departments of Anaesthesia and Biomedicine, Basel University Hospital, Hebelstrasse 20, 4031 Basel, Switzerland.
Leda BergamelliDepartment of Life Science and Biotechnology, University of Ferrara, Via Borsari 46, 44100, Ferrara, Italy.
Susan TrevesDepartment of Life Science and Biotechnology, University of Ferrara, Via Borsari 46, 44100, Ferrara, Italy From the Departments of Anaesthesia and Biomedicine, Basel University Hospital, Hebelstrasse 20, 4031 Basel, Switzerland.
Rossana BongianinoMolecular Cardiology Laboratories Fondazione Salvatore Maugeri, Via Maugeri 10/10°, 27100, Pavia Italy.
Marco De NegriMolecular Cardiology Laboratories Fondazione Salvatore Maugeri, Via Maugeri 10/10°, 27100, Pavia Italy.
Silvia G PrioriMolecular Cardiology Laboratories Fondazione Salvatore Maugeri, Via Maugeri 10/10°, 27100, Pavia Italy, Department of Molecular Medicine, University of Pavia, Pavia Italy, and.
Feliciano ProtasiCenter for Research on Ageing and Translational Medicine and DNICS (Department of Neuroscience, Imaging, and Clinical Sciences), University G. d'Annunzio, 66100 Chieti, Italy.
Francesco ZorzatoFrom the Departments of Anaesthesia and Biomedicine, Basel University Hospital, Hebelstrasse 20, 4031 Basel, Switzerland, Department of Life Science and Biotechnology, University of Ferrara, Via Borsari 46, 44100, Ferrara, Italy zor@unife.it.
Fondazione Salvatore Maugeri · ITUniversity of Ferrara · ITUniversity Hospital of Basel · CHUniversity of Chieti-Pescara · IT

Funding

Telethon GGP14003
6 · The paper itself

Abstract

We exploited a variety of mouse models to assess the roles of JP45-CASQ1 (CASQ, calsequestrin) and JP45-CASQ2 on calcium entry in slow twitch muscles. In flexor digitorum brevis (FDB) fibers isolated from JP45-CASQ1-CASQ2 triple KO mice, calcium transients induced by tetanic stimulation rely on calcium entry via La(3+)- and nifedipine-sensitive calcium channels. The comparison of excitation-coupled calcium entry (ECCE) between FDB fibers from WT, JP45KO, CASQ1KO, CASQ2KO, JP45-CASQ1 double KO, JP45-CASQ2 double KO, and JP45-CASQ1-CASQ2 triple KO shows that ECCE enhancement requires ablation of both CASQs and JP45. Calcium entry activated by ablation of both JP45-CASQ1 and JP45-CASQ2 complexes supports tetanic force development in slow twitch soleus muscles. In addition, we show that CASQs interact with JP45 at Ca(2+) concentrations similar to those present in the lumen of the sarcoplasmic reticulum at rest, whereas Ca(2+) concentrations similar to those present in the SR lumen after depolarization-induced calcium release cause the dissociation of JP45 from CASQs. Our results show that the complex JP45-CASQs is a negative regulator of ECCE and that tetanic force development in slow twitch muscles is supported by the dynamic interaction between JP45 and CASQs.

Indexed as

AnimalsCalciumCalcium-Binding ProteinsCalsequestrinGene Knockout TechniquesMembrane ProteinsMiceMuscle ContractionMuscle Fibers, Slow-TwitchMuscle, SkeletalSarcoplasmic ReticulumCalciumCalcium-Binding ProteinsCalsequestrinCasq1 protein, mousecasq2 protein, mouseJP-45 protein, mouseMembrane Proteinscalcium-binding proteinexcitation-contraction coupling (E-C coupling)gene knockoutmuscle physiologysarcoplasmic reticulum (SR)

Identifiers

PMID27189940
PMCPMC4938177
OpenAlexW2345616247

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.