ArticleMolecular biology of the cell2016
Iterative sorting of apical and basolateral cargo in Madin-Darby canine kidney cells.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Dirty Earth, Diseased Hearts: Soil, Water, and Plastic Pollution in the Cardiovascular Exposome.Anatolian journal of cardiology · 2026Article
- Soil and water pollution and cardiovascular disease.Nature reviews. Cardiology · 2025Review
- Antioxidant Potential ofAdvances in pharmacological and pharmaceutical sciences · 2025Article
- Impact of nanoparticles on human health and disease.Nepal journal of epidemiology · 2023Article
- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Low Rho activity in hepatocytes prevents apical from basolateral cargo separation during trans-Golgi network to surface transport.Traffic (Copenhagen, Denmark) · 2020Article
- Toxicity of Microplastics and Nanoplastics in Mammalian Systems.International journal of environmental research and public health · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
For several decades, the trans-Golgi network (TGN) was considered the most distal stop and hence the ultimate protein-sorting station for distinct apical and basolateral transport carriers that reach their respective surface domains in the direct trafficking pathway. However, recent reports of apical and basolateral cargoes traversing post-Golgi compartments accessible to endocytic ligands before their arrival at the cell surface and the post-TGN breakup of large pleomorphic membrane fragments that exit the Golgi region toward the surface raised the possibility that compartments distal to the TGN mediate or contribute to biosynthetic sorting. Here we describe the development of a novel assay that quantitatively distinguishes different cargo pairs by their degree of colocalization at the TGN and by the evolution of colocalization during their TGN-to-surface transport. Keys to the high resolution of our approach are 1) conversion of perinuclear organelle clustering into a two-dimensional microsomal spread and 2) identification of TGN and post-TGN cargo without the need for a TGN marker that universally cosegregates with all cargo. Using our assay, we provide the first evidence that apical NTRp75 and basolateral VSVG in Madin-Darby canine kidney cells still undergo progressive sorting after they exit the TGN toward the cell surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.