Evidence map›Paper›PMID 27289592›Full record

ArticlePlant cell reports2016

Single nucleotide polymorphism in sugar pathway and disease resistance genes in sugarcane.

Swarup K Parida, Sanjay Kalia, Awadhesh Pandit, Preetam Nayak, Ram Kushal Singh, Kishor Gaikwad, Prem Shankar Srivastava, Nagendra K Singh, Trilochan Mohapatra

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Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Swarup K ParidaNational Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Sanjay KaliaNational Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Awadhesh PanditNational Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Preetam NayakUtkal University, Vanivihar, Bhubaneswar, Odisha, 751004, India.
Ram Kushal SinghU.P. Council of Sugarcane Research, Shahjahanpur, Uttar Pradesh, 242001, India.
Kishor GaikwadNational Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Prem Shankar SrivastavaDepartment of Biotechnology, Jamia Hamdard University, New Delhi, 110062, India.
Nagendra K SinghNational Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Trilochan MohapatraNational Research Centre on Plant Biotechnology, New Delhi, 110012, India. tmnrcpb@gmail.com.
National Research Centre on Plant Biotechnology · INDepartment of Biotechnology · INUtkal University · INUttar Pradesh Council of sugarcane research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageSingle nucleotide polymorphism in sugar pathway and disease resistance genes showing genetic association with sugar content and red rot resistance would be useful in marker-assisted genetic improvement of sugarcane. Validation and genotyping of potential sequence variants in candidate genes are necessary to understand their functional significance and trait association potential. We discovered, characterized, validated and genotyped SNPs and InDels in sugar pathway and disease resistance genes of Saccharum complex and sugarcane varieties using amplicon sequencing and CAPS assays. The SNPs were abundant in the non-coding 3'UTRs than 5'UTRs and coding sequences depicting a strong bias toward C to T transition substitutions than transversions. Sequencing of cloned amplicons validated 61.6 and 45.2 % SNPs detected in silico in 21 sugar pathway and 16 disease resistance genes, respectively. Sixteen SNPs in four sugar pathway genes and 10 SNPs in nine disease resistance genes were validated through cost-effective CAPS assay. Functional and adaptive significance of SNP and protein haplotypes identified in sugar pathway and disease resistance genes was assessed by correlating their allelic variation with missense amino acid substitutions in the functional domains, alteration in protein structure models and possible modulation of catalytic enzyme activity in contrasting high and low sugar and moderately red rot resistant and highly susceptible sugarcane genotypes. A strong genetic association of five SNPs in the sugar pathway and disease resistance genes, and an InDel marker in the promoter sequence of sucrose synthase-2 gene, with sugar content and red rot resistance, was evident. The functionally relevant SNPs and InDels, detected and validated in sugar pathway and disease resistance genes, and genic CAPS markers designed, would be of immense use in marker-assisted genetic improvement of sugarcane for sugar content and disease resistance.

Indexed as

Genes, PlantBase SequenceCarbohydrate MetabolismDisease ResistanceGene Expression ProfilingGenetic MarkersGenotyping TechniquesHaplotypesINDEL MutationPlant DiseasesPolymorphism, Single NucleotideReproducibility of ResultsSaccharumSequence AlignmentGenetic MarkersCAPSDisease resistance genesSNPsSugarcaneSugar pathway genes

Identifiers

PMID27289592
OpenAlexW2415851375

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.