ArticleScientific reports2016
Using oriented peptide array libraries to evaluate methylarginine-specific antibodies and arginine methyltransferase substrate motifs.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The Identification of a Sub-Micromolar Peptide-Based Protein Arginine Methyltransferase 1 (PRMT1) Inhibitor from a Plate-Based Screening Assay.Biomolecules · 2025Article
- Uncovering Enzyme-Specific Post-Translational Modifications: An Overview of Current Methods.Proteomes · 2025Review
- Review
- PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.Cell metabolism · 2023Article
- Critical Roles of Protein Arginine Methylation in the Central Nervous System.Molecular neurobiology · 2023Review
- Specificity Analysis of Protein Methyltransferases and Discovery of Novel Substrates Using SPOT Peptide Arrays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Independent transcriptomic and proteomic regulation by type I and II protein arginine methyltransferases.iScience · 2021Article
- Large-scale identification of protein histidine methylation in human cells.NAR genomics and bioinformatics · 2021Article
- A Cyclic Peptide Epitope of an Under-Explored VEGF-B Loop 1 DemonstratedFrontiers in pharmacology · 2021Article
- Genetic evidence for partial redundancy between the arginine methyltransferases CARM1 and PRMT6.The Journal of biological chemistry · 2020Article
- Stability of tuberous sclerosis complex 2 is controlled by methylation at R1457 and R1459.Scientific reports · 2020Article
- CARM1 Regulates AMPK Signaling in Skeletal Muscle.iScience · 2020Article
- A Degenerate Peptide Library Approach to Reveal Sequence Determinants of Methyllysine-Driven Protein Interactions.Frontiers in cell and developmental biology · 2020Article
- A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity.Science advances · 2018Article
- Article
- CARM1 methylates MED12 to regulate its RNA-binding ability.Life science alliance · 2018Article
- Global mapping of CARM1 substrates defines enzyme specificity and substrate recognition.Nature communications · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Signal transduction in response to stimuli relies on the generation of cascades of posttranslational modifications that promote protein-protein interactions and facilitate the assembly of distinct signaling complexes. Arginine methylation is one such modification, which is catalyzed by a family of nine protein arginine methyltransferases, or PRMTs. Elucidating the substrate specificity of each PRMT will promote a better understanding of which signaling networks these enzymes contribute to. Although many PRMT substrates have been identified, and their methylation sites mapped, the optimal target motif for each of the nine PRMTs has not been systematically addressed. Here we describe the use of Oriented Peptide Array Libraries (OPALs) to methodically dissect the preferred methylation motifs for three of these enzymes - PRMT1, CARM1 and PRMT9. In parallel, we show that an OPAL platform with a fixed methylarginine residue can be used to validate the methyl-specific and sequence-specific properties of antibodies that have been generated against different PRMT substrates, and can also be used to confirm the pan nature of some methylarginine-specific antibodies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.