ArticleInternational journal of molecular sciences2016
Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Three Types of Rat Adipose Tissue.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Validation of Optimal Reference Genes for qRT-PCR in Adipose- and Uterine-Derived Feline Mesenchymal Stem Cells.Veterinary sciences · 2026Article
- A distal enhancer of Pparα regulates thermogenesis and mitochondrial function in brown fat.PLoS genetics · 2025Article
- Determination of the Roles ofBiology · 2025Article
- 5G Radiofrequency Exposure ReducesInternational journal of molecular sciences · 2025Article
- Human miR-1 Stimulates Metabolic and Thermogenic-Related Genes in Adipocytes.International journal of molecular sciences · 2024Article
- Cinnamon powder intake enhances the effect of caloric restriction on white adipose tissue in male rats.Journal of molecular histology · 2024Article
- Plant miR6262 Modulates the Expression of Metabolic and Thermogenic Genes in Human Hepatocytes and Adipocytes.Nutrients · 2024Article
- Identifying the stability of housekeeping genes to be used for the quantitative real-time PCR normalization in retinal tissue of streptozotocin-induced diabetic rats.International journal of ophthalmology · 2024Article
- Assessment of Reference Genes Stability in Cortical Bone of Obese and Diabetic Mice.Diabetes, metabolic syndrome and obesity : targets and therapy · 2024Article
- LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions.International journal of molecular sciences · 2023Article
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- Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice.Frontiers in nutrition · 2023Article
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- Partial Replacement of Dietary Fat with Polyunsaturated Fatty Acids Attenuates the Lipopolysaccharide-Induced Hepatic Inflammation in Sprague-Dawley Rats Fed a High-Fat Diet.International journal of environmental research and public health · 2021Article
- Optimizations for identifying reference genes in bone and cartilage bioengineering.BMC biotechnology · 2021Article
- Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice.Scientific reports · 2021Article
- Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.PloS one · 2021Article
- Biliverdin Reductase A (BVRA) Knockout in Adipocytes Induces Hypertrophy and Reduces Mitochondria in White Fat of Obese Mice.Biomolecules · 2020Article
- Hypoxia and autophagic response of obese adult rat adipocytes which differ in nutritional state during childhood.Journal of clinical biochemistry and nutrition · 2020Article
- High-Fat Diet Alters the Expression of Reference Genes in Male Mice.Frontiers in nutrition · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (qRT-PCR) is the most classical technique in the field of gene expression study. This method requires an appropriate reference gene to normalize mRNA levels. In this study, the expression stability of four frequently-used reference genes in epididymal white adipose tissue (eWAT), inguinal beige adipose tissue (iBeAT) and brown adipose tissue (BAT) from obese and lean rats were evaluated by geNorm, NormFinder and BestKeeper. Based on the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, the two most stable reference genes were recommended in each type of adipose tissue. Two target genes were applied to test the stability of the reference genes. The geNorm and NormFinder results revealed that GAPDH and 36B4 exhibited the highest expression stabilities in eWAT, while 36B4 and β-actin had the highest expression stabilities in iBeAT and BAT. According to the results of the BestKeeper analysis, 36B4 was the most stable gene in eWAT, iBeAT and BAT, in terms of the coefficient of variance. In terms of the coefficient of correlation, GAPDH, 36B4 and β-actin were the most stable genes in eWAT, iBeAT and BAT, respectively. Additionally, expected results and statistical significance were obtained using a combination of two suitable reference genes for data normalization. In conclusion, 36B4 and GAPDH, in combination, are the best reference genes for eWAT, while 36B4 and β-actin are two most suitable reference genes for both iBeAT and BAT. We recommend using these reference genes accordingly.
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