Evidence map›Paper›PMID 27344248›Full record

ArticleBiochimica et biophysica acta2016

Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism.

Vinay Sachdev, Christina Leopold, Raimund Bauer, Jay V Patankar, Jahangir Iqbal, Sascha Obrowsky, Renze Boverhof, Marcela Doktorova, Bernhard Scheicher, Madeleine Goeritzer and 7 more

Open access · hybridAbstract read
In one paragraph

Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.2field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 26 citations in OpenAlex.

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  9. Lysosomal acid lipase regulates fatty acid channeling in brown adipose tissue to maintain thermogenesis.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 8 institutions in 6 countries.

Vinay SachdevInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria.
Christina LeopoldInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria.
Raimund BauerInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria; Center of Experimental Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Jay V PatankarInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria; Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.
Jahangir IqbalDepartment of Cell Biology, SUNY Downstate Medical Center, 11203 New York, United States.
Sascha ObrowskyInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria.
Renze BoverhofDepartments of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Center Groningen, 9713 Groningen, The Netherlands.
Marcela DoktorovaDepartments of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Center Groningen, 9713 Groningen, The Netherlands.
Bernhard ScheicherInstitute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria.
Madeleine GoeritzerInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria.
Dagmar KolbInstitute of Cell Biology, Histology, and Embryology, Medical University of Graz, 8010 Graz, Austria.
Andrew V TurnbullAstraZeneca R&D, 431 50 Moelndal, Sweden.
Andreas ZimmerInstitute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria.
Gerald HoeflerInstitute of Pathology, Medical University of Graz, 8010 Graz, Austria.
M Mahmood HussainDepartment of Cell Biology, SUNY Downstate Medical Center, 11203 New York, United States.
Albert K GroenDepartments of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Center Groningen, 9713 Groningen, The Netherlands.
Dagmar KratkyInstitute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria. Electronic address: dagmar.kratky@medunigraz.at.
Medical University of Graz · ATSUNY Downstate Health Sciences University · USUniversity Medical Center Groningen · NLUniversity of Graz · ATAstraZeneca (Sweden) · SEUniversity Medical Center Hamburg-Eppendorf · DEUniversity of British Columbia · CAUniversity of Groningen · NL

Funding

Austrian Science Fund FWF F 3004Austrian Science Fund FWF P 22832Austrian Science Fund FWF P 27070Austrian Science Fund FWF W 1226
6 · The paper itself

Abstract

Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in triacylglycerol (TG) biosynthesis. Here we show that genetic deficiency and pharmacological inhibition of DGAT1 in mice alters cholesterol metabolism. Cholesterol absorption, as assessed by acute cholesterol uptake, was significantly decreased in the small intestine and liver upon DGAT1 deficiency/inhibition. Ablation of DGAT1 in the intestine (I-DGAT1(-/-)) alone is sufficient to cause these effects. Consequences of I-DGAT1 deficiency phenocopy findings in whole-body DGAT1(-/-) and DGAT1 inhibitor-treated mice. We show that deficiency/inhibition of DGAT1 affects cholesterol metabolism via reduced chylomicron size and increased trans-intestinal cholesterol excretion. These effects are independent of cholesterol uptake at the apical surface of enterocytes but mediated through altered dietary fatty acid metabolism. Our findings provide insight into a novel role of DGAT1 and identify a pathway by which intestinal DGAT1 deficiency affects whole-body cholesterol homeostasis in mice. Targeting intestinal DGAT1 may represent a novel approach for treating hypercholesterolemia.

Indexed as

AnimalsCholesterolDiacylglycerol O-AcyltransferaseDietary FatsFatty AcidsHypercholesterolemiaIntestinal AbsorptionLipid MetabolismLipogenesisLiverMiceTriglyceridesCholesterolDgat1 protein, mouseDiacylglycerol O-AcyltransferaseDietary FatsFatty AcidsTriglyceridesCholesterol absorptionChylomicron sizeDGAT1 inhibitionIntestinal DGAT1 deficiencyTrans-intestinal cholesterol excretion

Identifiers

PMID27344248
PMCPMC4948681
OpenAlexW2466685748

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.