ArticleExperimental cell research2016
Rab3A, a possible marker of cortical granules, participates in cortical granule exocytosis in mouse eggs.
Article in Experimental cell research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Endometriosis disrupts oocyte metabolism and cortical granule function, impairing fertilization.Reproductive biology and endocrinology : RB&E · 2026Article
- Phenogenetics of cortical granule dynamics during zebrafish oocyte-to-embryo transition.Frontiers in cell and developmental biology · 2025Review
- Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor.Scientific reports · 2024Article
- Maternal regulation of the vertebrate oocyte-to-embryo transition.PLoS genetics · 2024Article
- The light chain of tetanus toxin bound to arginine-rich cell-penetrating peptide inhibits cortical reaction in mouse oocytes.Frontiers in cell and developmental biology · 2023Article
- Phototherapeutic effect of transformable peptides containing pheophorbide a on colorectal cancer.Drug delivery · 2022Article
- Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes.Antioxidants (Basel, Switzerland) · 2022Article
- Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein.Small GTPases · 2022Article
- VAMPs sensitive to tetanus toxin are required for cortical granule exocytosis in mouse oocytes.Experimental cell research · 2021Article
- Knockin' on Egg's Door: Maternal Control of Egg Activation That Influences Cortical Granule Exocytosis in Animal Species.Frontiers in cell and developmental biology · 2021Review
- Modulation of P2Y2 receptors in bovine cumulus oocyte complexes: effects on intracellular calcium, zona hardening and developmental competence.Purinergic signalling · 2020Article
- Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice.Scientific reports · 2019Article
- SNAP23 is required for constitutive and regulated exocytosis in mouse oocytes†.Biology of reproduction · 2019Article
- Live imaging of cortical granule exocytosis reveals thatBiology open · 2018Article
- Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation.Journal of assisted reproduction and genetics · 2018Article
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Authors and funding
8 authors at 5 institutions in 3 countries.
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Abstract
Fusion of cortical granules with the oocyte plasma membrane is the most significant event to prevent polyspermy. This particular exocytosis, also known as cortical reaction, is regulated by calcium and its molecular mechanism is still not known. Rab3A, a member of the small GTP-binding protein superfamily, has been implicated in calcium-dependent exocytosis and is not yet clear whether Rab3A participates in cortical granules exocytosis. Here, we examine the involvement of Rab3A in the physiology of cortical granules, particularly, in their distribution during oocyte maturation and activation, and their participation in membrane fusion during cortical granule exocytosis. Immunofluorescence and Western blot analysis showed that Rab3A and cortical granules have a similar migration pattern during oocyte maturation, and that Rab3A is no longer detected after cortical granule exocytosis. These results suggested that Rab3A might be a marker of cortical granules. Overexpression of EGFP-Rab3A colocalized with cortical granules with a Pearson correlation coefficient of +0.967, indicating that Rab3A and cortical granules have almost a perfect colocalization in the egg cortical region. Using a functional assay, we demonstrated that microinjection of recombinant, prenylated and active GST-Rab3A triggered cortical granule exocytosis, indicating that Rab3A has an active role in this secretory pathway. To confirm this active role, we inhibited the function of endogenous Rab3A by microinjecting a polyclonal antibody raised against Rab3A prior to parthenogenetic activation. Our results showed that Rab3A antibody microinjection abolished cortical granule exocytosis in parthenogenetically activated oocytes. Altogether, our findings confirm that Rab3A might function as a marker of cortical granules and participates in cortical granule exocytosis in mouse eggs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.