Evidence map›Paper›PMID 27530762›Full record

ArticleNature communications2016

Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs.

Takashi Ohtsuki, Shigeto Kanzaki, Sae Nishimura, Yoshio Kunihiro, Masahiko Sisido, Kazunori Watanabe

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Optochemical Control of mTOR Signaling and mTOR-Dependent Autophagy.ACS pharmacology & translational science · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Takashi OhtsukiDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Shigeto KanzakiDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Sae NishimuraDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Yoshio KunihiroDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Masahiko SisidoDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Kazunori WatanabeDepartment of Biomedical Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Okayama University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.

Indexed as

LightProtein BiosynthesisAcylationAnimalsCHO CellsCodonCoumarinsCricetinaeCricetulusFrameshift MutationGreen Fluorescent ProteinsLasersLiposomesPeptide Elongation Factor TuPhotolysisRNA, MessengerCodoncoumarinCoumarinsenhanced green fluorescent proteinGreen Fluorescent ProteinsLiposomesPeptide Elongation Factor TuRNA, MessengerRNA, Transfer, Amino Acyl

Identifiers

PMID27530762
PMCPMC4992060
OpenAlexW2513261729

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.