Evidence map›Paper›PMID 27594697›Full record

ArticleBiochimica et biophysica acta2016

Acylation of lysine residues in human plasma high density lipoprotein increases stability and plasma clearance in vivo.

Yaliu Yang, Corina Rosales, Baiba K Gillard, Antonio M Gotto, Henry J Pownall

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Yaliu YangHouston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX 77030, USA; Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China. Electronic address: yyang3@houstonmethodist.org.
Corina RosalesHouston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX 77030, USA; Weill Cornell Medicine, 1305 York Avenue, New York, NY 10065, USA. Electronic address: crosales@houstonmethodist.org.
Baiba K GillardHouston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX 77030, USA; Weill Cornell Medicine, 1305 York Avenue, New York, NY 10065, USA. Electronic address: bgillard@houstonmethodist.org.
Antonio M GottoHouston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX 77030, USA; Weill Cornell Medicine, 1305 York Avenue, New York, NY 10065, USA. Electronic address: amg2004@med.cornell.edu.
Henry J PownallHouston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX 77030, USA; Weill Cornell Medicine, 1305 York Avenue, New York, NY 10065, USA. Electronic address: HJPownall@houstonmethodist.org.
Houston Methodist · US

Funding

Therapeutic Approaches to Dysregulated HDL MetabolismR01HL056865 · NHLBI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI POWNALL, HENRY J. · 1997 to 2015
$5.2M
High Density Lipoprotein Biogenesis and SpeciationR01HL129767 · NHLBI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI POWNALL, HENRY J., ROSALES, CORINA · 2016 to 2019
$2.3M
NHLBI NIH HHS R01 HL056865NHLBI NIH HHS R01 HL129767
6 · The paper itself

Abstract

Although human plasma high density lipoproteins (HDL) concentrations negatively correlate with atherosclerotic cardiovascular disease, underlying mechanisms are unknown. Thus, there is continued interest in HDL structure and functionality. Numerous plasma factors disrupt HDL structure while inducing the release of lipid free apolipoprotein (apo) AI. Given that HDL is an unstable particle residing in a kinetic trap, we tested whether HDL could be stabilized by acylation with acetyl and hexanoyl anhydrides, giving AcHDL and HexHDL respectively. Lysine analysis with fluorescamine showed that AcHDL and HexHDL respectively contained 11 acetyl and 19 hexanoyl groups. Tests with biological and physicochemical perturbants showed that HexHDL was more stable than HDL to perturbant-induced lipid free apo AI formation. Like the reaction of streptococcal serum opacity factor against HDL, the interaction of HDL with its receptor, scavenger receptor class B member 1 (SR-B1), removes CE from HDL. Thus, we tested and validated the hypothesis that selective uptake of HexHDL-[

Indexed as

AcylationAnimalsBileCHO CellsCholesterol EstersChromatography, GelCricetinaeCricetulusGallbladderHumansKineticsLipoproteins, HDLLiverLysineMice, Inbred C57BLMolecular WeightCholesterol EstersLipoproteins, HDLLysineopacity factorPeptide HydrolasesPhospholipid Transfer ProteinsAtherogenesisHigh density lipoprotein acylationHigh density lipoprotein stabilityReverse cholesterol transportScavenger receptor class B member 1

Identifiers

PMID27594697
PMCPMC5129619
OpenAlexW2516263556

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.