Evidence map›Paper›PMID 27611950›Full record

ArticleOncotarget2016

MicroRNA-21 promotes TGF-β1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression.

Chang Li, Lei Song, Zhuo Zhang, Xiao-Xue Bai, Ming-Fu Cui, Lian-Jun Ma

RetractedErratum issuedOpen access · diamondAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 32 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.

  1. Pooled it
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  6. Epigenetic Regulation of EMP/EMT-Dependent Fibrosis.International journal of molecular sciences · 2024
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  8. Clinical ophthalmology (Auckland, N.Z.) · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Chang LiDepartment of Gastrointestinal Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Lei SongDepartment of Respiratory Medicine, The First Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Zhuo ZhangDepartment of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Xiao-Xue BaiDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Ming-Fu CuiDepartment of Gastrointestinal Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Lian-Jun MaDepartment of Endoscopics, China-Japan Union Hospital of Jilin University, Changchun, 130000, Jilin, P.R. China.
Jilin University · CNFirst Hospital of Jilin University · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to explore the effects of miR-21 and PTEN/Akt signaling pathway on TGF-β1-induced epithelial-mesenchymal transition (EMT) in gastric cancer (GC). GC tissues and adjacent tissues were collected from 83 patients. The qRT-PCR assay was performed to detect miR-21 expression. The expressions of PTEN, Akt and p-Akt were detected by immunohistochemistry. After 48 h of treatment with TGF-β1 (10 ng/mL), the SGC-7901 and KATO-III cells were divided into the blank, negative control (NC), miR-21 inhibitors, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups. EMT related factors and PTEN expressions were detected by qRT-PCR assay and Western blotting. The scratch test was conducted to observe cell migration. Xenograft tumor model in nude mice was used to evaluate the effects of miR-21 on EMT of GC cells in vivo. In GC tissues, the expressions of miR-21, Akt and p-Akt were up-regulated, while PTEN expression was down-regulated. Gene and protein expressions of E-cadherin and PTEN in the miR-21 inhibitors group were higher than the blank, NC, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups, while the expressions of N-cadherin, β-catenin, Vimentin and Slug in the miR-21 inhibitors group were lower than other groups. MiR-21 inhibitors significantly inhibit cell migration and invasion in GC cell lines. In vivo xenograft experiment revealed that miR-21 inhibitor inhibits the growth of transplanted tumor through up-regulating E-cadherin and PTEN expressions and down-regulating the expressions of N-cadherin, β-catenin, Vimentin and Slug. These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression.

Indexed as

AdultAgedAnimalsEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHeterograftsHumansMaleMiceMice, NudeMicroRNAsMiddle AgedPTEN PhosphohydrolaseStomach NeoplasmsTransforming Growth Factor beta1MicroRNAsMIRN21 microRNA, humanPTEN PhosphohydrolasePTEN protein, humanTransforming Growth Factor beta1epithelial-mesenchymal transitiongastric cancerMicroRNA-21PTENTGF-β1

Identifiers

PMID27611950
PMCPMC5341852
OpenAlexW2508318947

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.