ArticleProceedings of the National Academy of Sciences of the United States of America1989
Osmoregulatory changes in myo-inositol transport by renal cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 135 citations in OpenAlex.
- Physiological roles of phosphoinositides and inositol phosphates: Implications for metabolic dysfunction-associated steatotic liver disease.Clinical science (London, England : 1979) · 2025Review
- Correlation between tooth decay and insulin resistance in normal weight males prompts a role for myo-inositol as a regenerative factor in dentistry and oral surgery: a feasibility study.Frontiers in bioengineering and biotechnology · 2024Article
- Review
- Structural and Functional Alterations in Betel-Quid Chewers: A Systematic Review of Neuroimaging Findings.Frontiers in psychiatry · 2019Article
- Metabolites Alterations in the Medial Prefrontal Cortex of Methamphetamine Users in Abstinence: AFrontiers in psychiatry · 2018Article
- Putative role of the H(+)/sucrose symporter SLC45A3 as an osmolyte transporter in the kidney.Pflugers Archiv : European journal of physiology · 2016Article
- Neurochemical abnormalities in anterior cingulate cortex on betel quid dependence: a 2D (1)H MRS investigation.American journal of translational research · 2015Article
- Article
- Article
- Macromolecular crowding regulates assembly of mRNA stress granules after osmotic stress: new role for compatible osmolytes.The Journal of biological chemistry · 2012Article
- Neurochemistry of drug action: insights from proton magnetic resonance spectroscopic imaging and their relevance to addiction.Annals of the New York Academy of Sciences · 2010Review
- Determination of transport stoichiometry for two cation-coupled myo-inositol cotransporters: SMIT2 and HMIT.The Journal of physiology · 2005Article
- Ts65Dn mouse, a Down syndrome model, exhibits elevated myo-inositol in selected brain regions and peripheral tissues.Neurochemical research · 2000Article
- Tyrosine kinase inhibitors and immunosuppressants perturb the myo-inositol but not the betaine cotransporter in isotonic and hypertonic MDCK cells.Kidney international · 1999Article
- Effect of osmolality and myo-inositol deprivation on the transport properties of myo-inositol in primary astrocyte cultures.Neurochemical research · 1997Article
- myo-Inositol is an osmolyte in rat liver macrophages (Kupffer cells) but not in RAW 264.7 mouse macrophages.The Biochemical journal · 1997Article
- Na+/myo-inositol transport is regulated by basolateral tonicity in Madin-Darby canine kidney cells.The Journal of clinical investigation · 1996Article
- Localization and rapid regulation of Na+/myo-inositol cotransporter in rat kidney.The Journal of clinical investigation · 1995Article
- Autocrine inhibition of Na+/K(+)-ATPase by nitric oxide in mouse proximal tubule epithelial cells.The Journal of clinical investigation · 1995Article
- The canine betaine gamma-amino-n-butyric acid transporter gene: diverse mRNA isoforms are regulated by hypertonicity and are expressed in a tissue-specific manner.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renal medullary cells contain high concentrations of myo-inositol, sorbitol, betaine, and glycerophosphocholine, whose levels vary with urinary osmolality. Accumulation of these "compatible" organic osmolytes is believed to help the cells osmoregulate in response to the high extracellular osmolality that occurs as part of the urinary concentrating mechanism. MDCK cells (a line from dog kidney) were previously shown to accumulate myo-inositol in response to increased medium osmolality. We demonstrate here that this accumulation requires the presence of myo-inositol in the medium, implying that the myo-inositol is not synthesized by the cells but rather is transported into them from the extracellular solution. The MDCK cells contain sodium-dependent myo-inositol transporters. Relative to isotonic controls, sodium-dependent myo-inositol uptake is higher in cells exposed to increased osmolality either acutely (1-7 days) or chronically (greater than 1 year). Transport is further enhanced when the cells are cultured in myo-inositol-free medium. The transport has both high- and low-affinity components. The observed changes in transport involve changes in maximal velocity of the high-affinity component but not in its Km. We conclude that renal cells can osmoregulate by changing the number (or, less likely, the transport turnover rate) of functioning sodium-dependent myo-inositol transporters.
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