ArticleScientific reports2016
A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 39 citations in OpenAlex.
- Transcriptional Reprogramming Deploys a Compartmentalized 'Timebomb' in Catharanthus roseus to Fend Off Chewing Herbivores.Plant, cell & environment · 2025Article
- Clinal Variation in Short Tandem Repeats Linked to Gene Expression in Sunflower (Biomolecules · 2024Article
- Statistical Genomics Analysis of Simple Sequence Repeats from the Paphiopedilum Malipoense Transcriptome Reveals Control Knob Motifs Modulating Gene Expression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The Potential Role of Genic-SSRs in Driving Ecological Adaptation Diversity inInternational journal of molecular sciences · 2024Article
- A newly identified β-amyrin synthase gene hypothetically involved in oleanane-saponin biosynthesis fromHeliyon · 2023Article
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- De Novo transcriptome assembly and differential expression analysis of catharanthus roseus in response to salicylic acid.Scientific reports · 2022Article
- An expanded role for the transcription factorFrontiers in plant science · 2022Article
- Rapid Genome-Wide Location-Specific Polymorphic SSR Marker Discovery in Black Pepper by GBS Approach.Frontiers in plant science · 2022Article
- Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip.Nucleic acids research · 2021Article
- Alleles of the GRF3-2A Gene in Wheat and Their Agronomic Value.International journal of molecular sciences · 2021Article
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- Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method inFrontiers in physiology · 2020Article
- GATA and Phytochrome Interacting Factor Transcription Factors Regulate Light-Induced Vindoline Biosynthesis inPlant physiology · 2019Article
- A Polymorphic (CT)Frontiers in genetics · 2019Article
- Article
- Skewing of the genetic architecture at the ZMYM3 human-specific 5' UTR short tandem repeat in schizophrenia.Molecular genetics and genomics : MGG · 2018Article
- The effects of promoter variations of the N-Methylcanadine 1-Hydroxylase (CYP82Y1) gene on the noscapine production in opium poppy.Scientific reports · 2018Article
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- Molecular Cytogenetics ofBioMed research international · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Simple Sequence Repeats (SSRs) of polypurine-polypyrimidine type motifs occur very frequently in the 5' flanks of genes in plants and have recently been implicated to have a role in regulation of gene expression. In this study, 2 accessions of Catharanthus roseus having (CT)8 and (CT)21 varying motifs in the 5'UTR of Tryptophan decarboxylase (Tdc) gene, were investigated for its role in regulation of gene expression. Extensive Tdc gene expression analysis in the 2 accessions was carried out both at the level of transcription and translation. Transcript abundance was estimated using Northern analysis and qRT-PCR, whereas the rate of Tdc gene transcription was assessed using in-situ nuclear run-on transcription assay. Translation status of Tdc gene was monitored by quantification of polysome associated Tdc mRNA using qRT-PCR. These observations were validated through transient expression analysis using the fusion construct [CaM35S:(CT)8-21:GUS]. Our study demonstrated that not only does the length of (CT)n -SSRs influences the promoter activity, but the presence of SSRs per se in the 5'-UTR significantly enhances the level of gene expression. We termed this phenomenon as "microsatellite mediated enhancement" (MME) of gene expression. Results presented here will provide leads for engineering plants with enhanced amounts of medicinally important alkaloids.
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