Evidence map›Paper›PMID 27631978›Full record

ArticlePloS one2016

Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection.

Sarah Van Lent, Winnok H De Vos, Heather Huot Creasy, Patricia X Marques, Jacques Ravel, Daisy Vanrompay, Patrik Bavoil, Ru-Ching Hsia

Open access · goldAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. International journal of molecular sciences · 2020
    Article
  4. Article
  5. Chlamydial Infection From Outside to Inside.Frontiers in microbiology · 2019
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Sarah Van LentDepartment of Animal Production, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Winnok H De VosDepartment of Veterinary Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
Heather Huot CreasyInstitute for Genome Sciences and Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, Maryland, Unites States of America.
Patricia X MarquesDepartment of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, Maryland, Unites States of America.
Jacques RavelInstitute for Genome Sciences and Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, Maryland, Unites States of America.
Daisy VanrompayDepartment of Animal Production, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Patrik BavoilDepartment of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, Maryland, Unites States of America.
Ru-Ching HsiaUniversity of Maryland, Baltimore, Electron Microscopy Core Imaging Facility, Maryland, Unites States of America.
University of Maryland, Baltimore · USGhent University · BE

Funding

Serologic Biomarkers of Human Sexually Transmitted Infection and DiseaseU19AI084044 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI BAVOIL, PATRIK M · 2009 to 2018
$22.8M
NIAID NIH HHS U19 AI084044
6 · The paper itself

Abstract

The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels, protein production and localization of several Pmps of C. psittaci. Pmp expression profiles were characterized using quantitative real-time PCR (RT-qPCR), immunofluorescence (IF) and immuno-electron microscopy (IEM) under normal and stress conditions. We found that PmpA was highly produced in all inclusions as early as 12 hpi in all biological replicates. In addition, PmpA and PmpH appeared to be unusually accessible to antibody as determined by both immunofluorescence and immuno-electron microscopy. Our results suggest an important role for these Pmps in the pathogenesis of C. psittaci, and make them promising candidates in vaccine development.

Indexed as

Bacterial Outer Membrane ProteinsChlamydia InfectionsChlamydophila psittaciCloning, MolecularGene Expression ProfilingGenes, BacterialHeLa CellsHumansMicroscopy, ImmunoelectronBacterial Outer Membrane Proteins

Identifiers

PMID27631978
PMCPMC5025070
OpenAlexW2519209041

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.