ArticleScientific reports2016
A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 56 citations in OpenAlex.
- Characterization of a novel D-sorbitol dehydrogenase from Faunimonas pinastri A52C2.Applied microbiology and biotechnology · 2025Article
- Regeneration of cofactor NAD(P)Frontiers in bioengineering and biotechnology · 2025Review
- N-acylhomoserine lactonase-based hybrid nanoflowers: a novel and practical strategy to control plant bacterial diseases.Journal of nanobiotechnology · 2022Article
- Immobilization of Laccase Through Inorganic-Protein Hybrids Using Various Metal Ions.Indian journal of microbiology · 2022Article
- Enhanced production of l-sorbose by systematic engineering of dehydrogenases inSynthetic and systems biotechnology · 2022Article
- Nano-Therapeutics to Treat Acne Vulgaris.Indian journal of microbiology · 2022Review
- Anaerobic Digestion of Agri-Food Wastes for Generating Biofuels.Indian journal of microbiology · 2021Review
- Nano-Biocatalysts: Potential Biotechnological Applications.Indian journal of microbiology · 2021Review
- Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade.RSC advances · 2021Article
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- Identification of Gradient Promoters ofFrontiers in bioengineering and biotechnology · 2021Article
- Selective oxidation of alkyl and aryl glyceryl monoethers catalysed by an engineered and immobilised glycerol dehydrogenase.Chemical science · 2020Article
- Solvent-Tolerant Acyltransferase fromIndian journal of microbiology · 2019Article
- Identification of NAD-Dependent Xylitol Dehydrogenase fromACS omega · 2019Article
- Antimicrobial Activity of Biosynthesized Silver Nanoparticles Decorated Silica Nanoparticles.Indian journal of microbiology · 2019Article
- Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids.Indian journal of microbiology · 2019Article
- Cloning, Expression and Characterization of a Highly Active Alcohol Dehydrogenase for Production of Ethyl (S)-4-Chloro-3-Hydroxybutyrate.Indian journal of microbiology · 2019Article
- Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme.Indian journal of microbiology · 2019Article
- Overcoming NADPH product inhibition improves D-sorbitol conversion to L-sorbose.Scientific reports · 2019Article
- Nanoparticles in Biological Hydrogen Production: An Overview.Indian journal of microbiology · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sorbitol dehydrogenase (GoSLDH) from Gluconobacter oxydans G624 (G. oxydans G624) was expressed in Escherichia coli BL21(DE3)-CodonPlus RIL. The complete 1455-bp codon-optimized gene was amplified, expressed, and thoroughly characterized for the first time. GoSLDH exhibited Km and kcat values of 38.9 mM and 3820 s(-1) toward L-sorbitol, respectively. The enzyme exhibited high preference for NADP(+) (vs. only 2.5% relative activity with NAD(+)). GoSLDH sequencing, structure analyses, and biochemical studies, suggested that it belongs to the NADP(+)-dependent polyol-specific long-chain sorbitol dehydrogenase family. GoSLDH is the first fully characterized SLDH to date, and it is distinguished from other L-sorbose-producing enzymes by its high activity and substrate specificity. Isothermal titration calorimetry showed that the protein binds more strongly to D-sorbitol than other L-sorbose-producing enzymes, and substrate docking analysis confirmed a higher turnover rate. The high oxidation potential of GoSLDH for D-sorbitol was confirmed by cyclovoltametric analysis. Further, stability of GoSLDH significantly improved (up to 13.6-fold) after cross-linking of immobilized enzyme on silica nanoparticles and retained 62.8% residual activity after 10 cycles of reuse. Therefore, immobilized GoSLDH may be useful for L-sorbose production from D-sorbitol.
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