Evidence map›Paper›PMID 27638543›Full record

ArticleGenome research2016

RNA-DNA sequence differences in Saccharomyces cerevisiae.

Isabel X Wang, Christopher Grunseich, Youree G Chung, Hojoong Kwak, Girish Ramrattan, Zhengwei Zhu, Vivian G Cheung

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. RNA abasic sites in yeast and human cells.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  7. Article
  8. Article
  9. Article
  10. A-to-I mRNA editing in fungi: occurrence, function, and evolution.Cellular and molecular life sciences : CMLS · 2019
    Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Isabel X WangLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Christopher GrunseichNeurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
Youree G ChungCollege of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Hojoong KwakLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Girish RamrattanLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Zhengwei ZhuLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Vivian G CheungLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
Howard Hughes Medical Institute · USUniversity of Michigan · USNational Institutes of Health · US

Funding

Howard Hughes Medical Institute
6 · The paper itself

Abstract

Alterations of RNA sequences and structures, such as those from editing and alternative splicing, result in two or more RNA transcripts from a DNA template. It was thought that in yeast, RNA editing only occurs in tRNAs. Here, we found that Saccharomyces cerevisiae have all 12 types of RNA-DNA sequence differences (RDDs) in the mRNA. We showed these sequence differences are propagated to proteins, as we identified peptides encoded by the RNA sequences in addition to those by the DNA sequences at RDD sites. RDDs are significantly enriched at regions with R-loops. A screen of yeast mutants showed that RDD formation is affected by mutations in genes regulating R-loops. Loss-of-function mutations in ribonuclease H, senataxin, and topoisomerase I that resolve RNA-DNA hybrids lead to increases in RDD frequency. Our results demonstrate that RDD is a conserved process that diversifies transcriptomes and proteomes and provide a mechanistic link between R-loops and RDDs.

Indexed as

Base Pair MismatchDNA, FungalDNA Topoisomerases, Type ILoss of Function MutationRibonuclease HRNA, FungalRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsDNA, FungalDNA Topoisomerases, Type IRibonuclease HRNA, FungalRNA, MessengerSaccharomyces cerevisiae Proteins

Identifiers

PMID27638543
PMCPMC5088596
OpenAlexW2520772926

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.