Evidence map›Paper›PMID 27733157›Full record

ArticleCardiovascular diabetology2016

N-acetylcysteine attenuates myocardial dysfunction and postischemic injury by restoring caveolin-3/eNOS signaling in diabetic rats.

Wating Su, Yuan Zhang, Qiongxia Zhang, Jinjin Xu, Liying Zhan, Qiqi Zhu, Qingquan Lian, Huimin Liu, Zhong-Yuan Xia, Zhengyuan Xia and 1 more

Open access · goldAbstract read
In one paragraph

Article in Cardiovascular diabetology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 60 citations in OpenAlex.

  1. Role of membrane microdomains in cardiac protection: strategies for diabetic cardiomyopathy.American journal of physiology. Heart and circulatory physiology · 2025
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  14. Antioxidants (Basel, Switzerland) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Wating SuDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Yuan ZhangDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Qiongxia ZhangDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Jinjin XuDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Liying ZhanDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Qiqi ZhuDepartment of Anesthesiology, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Qingquan LianDepartment of Anesthesiology, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Huimin LiuDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Zhong-Yuan XiaDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
Zhengyuan XiaDepartment of Anesthesiology, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. zyxia@hku.hk.ORCID 0000-0002-7002-5524
Shaoqing LeiDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China. leishaoqing@163.com.
Wuhan University · CNWenzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPatients with diabetes are prone to develop cardiac hypertrophy and more susceptible to myocardial ischemia-reperfusion (I/R) injury, which are concomitant with hyperglycemia-induced oxidative stress and impaired endothelial nitric oxide (NO) synthase (eNOS)/NO signaling. Caveolae are critical in the transduction of eNOS/NO signaling in cardiovascular system. Caveolin (Cav)-3, the cardiomyocytes-specific caveolae structural protein, is decreased in the diabetic heart in which production of reactive oxygen species are increased. We hypothesized that treatment with antioxidant N-acetylcysteine (NAC) could enhance cardiac Cav-3 expression and attenuate caveolae dysfunction and the accompanying eNOS/NO signaling abnormalities in diabetes.

methodsControl or streptozotocin-induced diabetic rats were either untreated or treated with NAC (1.5 g/kg/day, NAC) by oral gavage for 4 weeks. Rats in subgroup were randomly assigned to receive 30 min of left anterior descending artery ligation followed by 2 h of reperfusion. Isolated rat cardiomyocytes or H9C2 cells were exposed to low glucose (LG, 5.5 mmol/L) or high glucose (HG, 25 mmol/L) for 36 h before being subjected to 4 h of hypoxia followed by 4 h of reoxygenation (H/R).

resultsNAC treatment ameliorated myocardial dysfunction and cardiac hypertrophy, and attenuated myocardial I/R injury and post-ischemic cardiac dysfunction in diabetic rats. NAC attenuated the reductions of NO, Cav-3 and phosphorylated eNOS and mitigated the augmentation of O

conclusionsHyperglycemia-induced inhibition of eNOS activity might be consequences of caveolae dysfunction and reduced Cav-3 expression. Antioxidant NAC attenuated myocardial dysfunction and myocardial I/R injury by improving Cav-3/eNOS signaling.

Indexed as

AcetylcysteineAnimalsAntioxidantsCardiomegalyCaveolaeCaveolin 3Cell HypoxiaCell LineCytoprotectionDiabetes Mellitus, ExperimentalDiabetic CardiomyopathiesHeart RateMaleMyocardial Reperfusion InjuryMyocytes, CardiacNitric OxideAcetylcysteineAntioxidantsCav3 protein, ratCaveolin 3Nitric OxideNitric Oxide Synthase Type IIINos3 protein, ratStreptozocinCaveolin-3DiabetesDiabetic cardiomyopathyMyocardial ischemia–reperfusion injuryN-acetylcysteine

Identifiers

PMID27733157
PMCPMC5062884
OpenAlexW2529864301

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.