Evidence map›Paper›PMID 27746176›Full record

ArticleBiochemical and biophysical research communications2017

Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor.

Yonggang Zhang, Fang Li, Xiao Xiao, Wu Deng, Chaoran Yin, Ting Zhang, Karnam S Murthy, Wenhui Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Association Analysis Between SNPs in the Promoter Region ofNeuropsychiatric disease and treatment · 2020
    Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yonggang ZhangDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA.
Fang LiDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA.
Xiao XiaoDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA.
Wu DengDepartment of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, 1101 East Marshall Street, Richmond, VA 23298, USA.
Chaoran YinDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA.
Ting ZhangDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA.
Karnam S MurthyDepartment of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, 1101 East Marshall Street, Richmond, VA 23298, USA.
Wenhui HuDepartment of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, USA. Electronic address: whu@temple.edu.
Temple University · USVirginia Commonwealth University Medical Center · US

Funding

HORMONAL INTEGRATION OF EXOCRINE AND ENDOCRINE ORGANSR01DK015564 · NIDDK · VIRGINIA COMMONWEALTH UNIVERSITY · PI KARNAM, S MURTHY · 1986 to 2020
$6.7M
Regulation of gut smooth muscle contraction and relaxation by cytokinesR01DK075964 · NIDDK · VIRGINIA COMMONWEALTH UNIVERSITY · PI HU, WENHUI · 2007 to 2016
$2.9M
NIDDK NIH HHS R01 DK015564NIDDK NIH HHS R01 DK075964
6 · The paper itself

Abstract

GATA transcription factors regulate an array of genes important in cell proliferation and differentiation. Here we report the identification of regulator of G protein signaling 4 (RGS4) as a novel target for GATA-6 transcription factor. Although three sites (a, b, c) within the proximal region of rabbit RGS4 promoter for GATA transcription factors were predicted by bioinformatics analysis, only GATA-a site (16 bp from the core TATA box) is essential for RGS4 transcriptional regulation. RT-PCR analysis demonstrated that only GATA-6 was highly expressed in rabbit colonic smooth muscle cells but GATA-4/6 were expressed in cardiac myocytes and GATA-1/2/3 expressed in blood cells. Adenovirus-mediated expression of GATA-6 but not GATA-1 significantly increased the constitutive and IL-1β-induced mRNA expression of the endogenous RGS4 in colonic smooth muscle cells. IL-1β stimulation induced GATA-6 nuclear translocation and increased GATA-6 binding to RGS4 promoter. These data suggest that GATA factor could affect G protein signaling through regulating RGS4 expression, and GATA signaling may develop as a future therapeutic target for RGS4-related diseases.

Indexed as

Active Transport, Cell NucleusAnimalsBase SequenceBinding SitesCells, CulturedColonGATA1 Transcription FactorGATA6 Transcription FactorGene Expression RegulationInterleukin-1betaMutagenesis, Site-DirectedMyocytes, Smooth MusclePromoter Regions, GeneticRabbitsRGS ProteinsRNA, MessengerGATA1 Transcription FactorGATA6 Transcription FactorInterleukin-1betaRGS4 proteinRGS ProteinsRNA, MessengerGastrointestinal tractGATA transcription factorsGene regulationsRegulator of G protein signalingSmooth muscle cells

Identifiers

PMID27746176
PMCPMC5266638
OpenAlexW2529993906

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.