ArticleShock (Augusta, Ga.)2017
Erythrocyte-Derived Microparticles Activate Pulmonary Endothelial Cells in a Murine Model of Transfusion.
Article in Shock (Augusta, Ga.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Case Report: Coexisting Hereditary Spherocytosis, Limited Cutaneous Systemic Sclerosis, and Antiphospholipid Syndrome: A Rare Autoimmune-Hemolytic Association.International journal of rheumatic diseases · 2026Article
- Extracellular Vesicles as Drivers of Lung Endothelial Dysfunction in ARDS: Mechanisms and Therapeutic Opportunities.Comprehensive Physiology · 2026Review
- Evaluation of the protective effect of direct peritoneal resuscitation on lung injury in a controlled hemorrhagic shock model in rats.Scientific reports · 2026Article
- Inflammatory lung diseases: a clinical and scientific review of the latest advances and challenges.Pharmacological reports : PR · 2025Review
- Article
- Adverse effects of microparticles on transfusion of stored red blood cell concentrates.Hematology, transfusion and cell therapy · 2024Article
- Porcine Packed Red Blood Cells Demonstrate a Distinct Red Blood Cell Storage Lesion.The Journal of surgical research · 2024Article
- Microvesicles from stored red blood cells induce P-selectin and von Willebrand factor release from endothelial cells via a protein kinase C-dependent mechanism.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Article
- Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.Transfusion · 2023Article
- Article
- Improving packed red blood cell storage with a high-viscosity buffered storage solution.Surgery · 2022Article
- Extracellular Vesicles Derived from Intermittent Hypoxia-Treated Red Blood Cells Impair Endothelial Function Through Regulating eNOS Phosphorylation and ET-1 Expression.Cardiovascular drugs and therapy · 2021Article
- Article
- Innate coagulability changes with age in stored packed red blood cells.Thrombosis research · 2020Article
- Contrasting effects of stored allogeneic red blood cells and their supernatants on permeability and inflammatory responses in human pulmonary endothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Microparticles from aged packed red blood cell units stimulate pulmonary microthrombus formation via P-selectin.Thrombosis research · 2020Article
- Complement C5a Induces Pro-inflammatory Microvesicle Shedding in Severely Injured Patients.Frontiers in immunology · 2020Article
- Microparticles formed during storage of red blood cell units support thrombin generation.The journal of trauma and acute care surgery · 2018Article
- Endocytosis of Red Blood Cell Microparticles by Pulmonary Endothelial Cells is Mediated By Rab5.Shock (Augusta, Ga.) · 2018Article
- Microparticles from stored red blood cells promote a hypercoagulable state in a murine model of transfusion.Surgery · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Erythrocyte-derived microparticles (MPs) are sub-micrometer, biologically active vesicles shed by red blood cells as part of the biochemical changes that occur during storage. We hypothesized that MPs from stored red blood cells would activate endothelial cells. MPs from aged murine packed red blood cells (pRBCs) were isolated and used to treat confluent layers of cultured endothelial cells. Endothelial expression of leukocyte adhesion molecules, endothelial-leukocyte adhesion molecule-1 (ELAM-1) and intercellular adhesion molecule-1(ICAM-1), and inflammatory mediator, interleukin-6 (IL-6), was evaluated at 0.5, 6, 12, and 24 h of treatment. Healthy C57BL/6 mice were transfused with a MP suspension and lung sections were analyzed for adhesion molecules and sequestered interstitial leukocytes. Increased levels of ELAM-1 and ICAM-1 were found on cultured endothelial cells 6 h after MP stimulation (6.91 vs. 4.07 relative fluorescent intensity [RFI], P < 0.01, and 5.85 vs. 3.55 RFI, P = 0.01, respectively). IL-6 in cell culture supernatants was increased after 12 h of MP stimulation compared with controls (1.24 vs. 0.73 ng/mL, P = 0.03). In vivo experiments demonstrated that MP injection increased ELAM-1 and ICAM-1 expression at 1 h (18.56 vs. 7.08 RFI, P < 0.01, and 23.66 vs. 6.87 RFI, P < 0.01, respectively) and caused increased density of pulmonary interstitial leukocytes by 4 h of treatment (69.25 vs. 29.25 cells/high powered field, P < 0.01). This series of experiments supports our hypothesis that erythrocyte-derived MPs are able to activate pulmonary endothelium, leading to the pulmonary sequestration of leukocytes following the transfusion of stored pRBCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.