Evidence map›Paper›PMID 27824296›Full record

ArticleEpigenetics2017

Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity.

Thanh V Hoang, Evan R Horowitz, Blake R Chaffee, Peipei Qi, Rachel E Flake, Devin G Bruney, Blake J Rasor, Savana E Rosalez, Brad D Wagner, Michael L Robinson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 19 citations in OpenAlex.

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  15. The lens growth process.Progress in retinal and eye research · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Thanh V Hoanga Department of Biology , Miami University , Oxford , OH , USA.
Evan R Horowitza Department of Biology , Miami University , Oxford , OH , USA.
Blake R Chaffeea Department of Biology , Miami University , Oxford , OH , USA.
Peipei Qia Department of Biology , Miami University , Oxford , OH , USA.
Rachel E Flakea Department of Biology , Miami University , Oxford , OH , USA.
Devin G Bruneya Department of Biology , Miami University , Oxford , OH , USA.
Blake J Rasora Department of Biology , Miami University , Oxford , OH , USA.
Savana E Rosaleza Department of Biology , Miami University , Oxford , OH , USA.
Brad D Wagnera Department of Biology , Miami University , Oxford , OH , USA.
Michael L Robinsona Department of Biology , Miami University , Oxford , OH , USA.
Miami University · US

Funding

Epigenetic regulation of lens fiber cell differentiation: The role of DNA methylaR21EY022210 · NEI · MIAMI UNIVERSITY OXFORD · PI ROBINSON, MICHAEL L · 2012 to 2013
$382k
NEI NIH HHS R21 EY022210
6 · The paper itself

Abstract

Despite the wealth of knowledge of transcription factors involved in lens development, little information exists about the role of DNA methylation in this process. Here, we investigated the role of DNA methylation in lens development and fiber cell differentiation using mice conditionally lacking maintenance or de novo methyltransferases in the lens lineage. We found that while Dnmt1 inactivation at the lens placode stage (via the Le-Cre transgene) led to lens DNA hypomethylation and severe lens epithelial apoptosis, lens fiber cell differentiation remained largely unaffected. The simultaneous deletion of phosphatase and tensin homolog (Pten) elevated the level of phosphorylated AKT and rescued many of the morphological defects and cell death in DNMT1-deficient lenses. With a different Cre driver (MLR10) we demonstrated that a small number of lens epithelial cells escaped Dnmt1-deletion and over-proliferated to compensate for the loss of Dnmt1-deleted cells, suggesting that lens epithelium possess a substantial capacity for self-renewal. Unlike lenses deficient for Dnmt1, inactivation of both Dnmt3a and Dnmt3b by either the Le-Cre or MLR10-Cre transgene did not result in any obvious lens phenotype prior to 10 months of age. Taken together, while lens epithelial cell survival requires DNMT1, morphologically normal lenses develop in the absence of both DNMT3A and DNMT3B.

Indexed as

AnimalsAnimals, NewbornCell DifferentiationDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3ADNA Methyltransferase 3BEmbryo, MammalianFemalegamma-CrystallinsGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicLens, CrystallineMiceMice, TransgenicDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADNA Methyltransferase 3BDnmt1 protein, mouseDnmt3a protein, mousegamma-CrystallinsDevelopmentDNA methylationDnmt1Dnmt3aDnmt3blens

Identifiers

PMID27824296
PMCPMC5270636
OpenAlexW2554484512

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.