ArticleScientific reports2016
Internal epitope tagging informed by relative lack of sequence conservation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- EpicTope: predicting and validating non-disruptive epitope tagging sites.Development (Cambridge, England) · 2026Article
- Epicardial Tcf21 facilitates cardiomyocyte dedifferentiation and heart regeneration in zebrafish.Developmental biology · 2026Article
- Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo.Nature communications · 2025Article
- Gap-junction-mediated bioelectric signaling required for slow muscle development and function in zebrafish.Current biology : CB · 2024Article
- Gap junction mediated bioelectric coordination is required for slow muscle development, organization, and function.bioRxiv : the preprint server for biology · 2023Article
- The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth.Disease models & mechanisms · 2023Article
- A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening.BMC genomics · 2022Article
- Generation of a zebrafish knock-in line expressing MYC-tagged Sox11a using CRISPR/Cas9 genome editing.Biochemical and biophysical research communications · 2022Article
- Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes.Scientific reports · 2020Article
- Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor.Frontiers in cell and developmental biology · 2020Article
- Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?Frontiers in molecular biosciences · 2020Review
- Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish.PLoS genetics · 2018Article
- Zebrafish knock-ins swim into the mainstream.Disease models & mechanisms · 2018Article
- Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9.Nucleic acids research · 2018Article
- Sequence-based prediction of permissive stretches for internal protein tagging and knockdown.BMC biology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.