Evidence map›Paper›PMID 27903645›Full record

ArticleGenome research2017

Upgrading short-read animal genome assemblies to chromosome level using comparative genomics and a universal probe set.

Joana Damas, Rebecca O'Connor, Marta Farré, Vasileios Panagiotis E Lenis, Henry J Martell, Anjali Mandawala, Katie Fowler, Sunitha Joseph, Martin T Swain, Darren K Griffin and 1 more

Abstract read
In one paragraph

Article in Genome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed.

  1. A cytogenetic analysis with chromosome painting in typical antbirds (Passeriformes, Thamnophilidae) and an inference on chromosome evolution in Passeriformes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
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  15. Evolution of the ancestral mammalian karyotype and syntenic regions.Proceedings of the National Academy of Sciences of the United States of America · 2022
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  19. Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022
    Review
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Joana DamasDepartment of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, NW1 0TU, United Kingdom.ORCID 0000-0003-4857-2510
Rebecca O'ConnorSchool of Biosciences, University of Kent, Canterbury, CT2 7NY, United Kingdom.
Marta FarréDepartment of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, NW1 0TU, United Kingdom.ORCID 0000-0001-9170-5767
Vasileios Panagiotis E LenisInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 3DA, United Kingdom.
Henry J MartellSchool of Biosciences, University of Kent, Canterbury, CT2 7NY, United Kingdom.
Anjali MandawalaSchool of Biosciences, University of Kent, Canterbury, CT2 7NY, United Kingdom.
Katie FowlerSchool of Human and Life Sciences, Canterbury Christ Church University, Canterbury, CT1 1QU, United Kingdom.
Sunitha JosephSchool of Biosciences, University of Kent, Canterbury, CT2 7NY, United Kingdom.
Martin T SwainInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 3DA, United Kingdom.
Darren K GriffinSchool of Biosciences, University of Kent, Canterbury, CT2 7NY, United Kingdom.
Denis M LarkinDepartment of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, NW1 0TU, United Kingdom.

Funding

Biotechnology and Biological Sciences Research Council BB/E010652/1Biotechnology and Biological Sciences Research Council BB/E024211/1Biotechnology and Biological Sciences Research Council BB/J010170/1Biotechnology and Biological Sciences Research Council BB/K008161/1Biotechnology and Biological Sciences Research Council BB/K008226/1Biotechnology and Biological Sciences Research Council G19438/2
6 · The paper itself

Abstract

Most recent initiatives to sequence and assemble new species' genomes de novo fail to achieve the ultimate endpoint to produce contigs, each representing one whole chromosome. Even the best-assembled genomes (using contemporary technologies) consist of subchromosomal-sized scaffolds. To circumvent this problem, we developed a novel approach that combines computational algorithms to merge scaffolds into chromosomal fragments, PCR-based scaffold verification, and physical mapping to chromosomes. Multigenome-alignment-guided probe selection led to the development of a set of universal avian BAC clones that permit rapid anchoring of multiple scaffolds to chromosomes on all avian genomes. As proof of principle, we assembled genomes of the pigeon (

Indexed as

GenomeAnimalsAvian ProteinsChromosome BreakpointsChromosomesColumbidaeConserved SequenceContig MappingFalconiformesGenomicsReference StandardsAvian Proteins

Identifiers

PMID27903645
PMCPMC5411781

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.