Evidence mapPaperPMID 27922698Full record

ArticleMolecular medicine reports2017

Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models.

Yunpeng Wang, Suying Wu, Yang Yang, Fen Peng, Qintao Li, Peng Tian, Erying Xiang, Honglu Liang, Beibei Wang, Xiaoyu Zhou and 2 more

Open access · hybridAbstract read
In one paragraph

Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Yunpeng WangDepartment of Neonatology, Nanshan People's Hospital, Affiliated to Guangdong Medical University, Shenzhen, Guangdong 518052, P.R. China.
Suying WuDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Yang YangDepartment of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Fen PengDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Qintao LiDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Peng TianDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Erying XiangDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Honglu LiangDepartment of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Beibei WangDepartment of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Xiaoyu ZhouDepartment of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Hua HuangDepartment of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China.
Xiaoguang ZhouDepartment of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Minda Hospital · CNNanjing Medical University · CNSecond Affiliated Hospital of Nanjing Medical University · CNShenZhen People’s Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study aimed to identify microRNAs (miRNAs) involved in regulating retinal neovascularization and retinopathy of prematurity (ROP). A total of 80 healthy C57BL/6 neonatal mice were randomly divided into the oxygen‑induced retinopathy (OIR) group (n=40), in which 7‑day‑old mice were maintained in 75% oxygen conditions for 5 days, or the control group (n=40). Following collection of retinal tissue, retinal angiography and hematoxylin and eosin (H&E) staining were performed. Total RNA was also extracted from retinal tissue, and miRNA microarrays and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were performed to identify differentially expressed miRNAs in the two groups. Retinal angiography and H&E staining revealed damage to retinas in the OIR group. Compared with the control group, 67 miRNAs were differentially expressed in the OIR group, of which 34 were upregulated and 33 were downregulated. Of these differentially expressed miRNAs, 32 exhibited a fold change ≥2, of which 21 were upregulated and 11 were downregulated. The results of RT‑qPCR for miR‑130a‑3p and miR‑5107‑5p were in accordance with those of the miRNA microarray. The newly identified miRNAs may be important in the development of ROP, and may provide a basis for future research into the mechanisms of ROP.

Indexed as

Gene Expression RegulationTranscriptomeAnimalsAnimals, NewbornFemaleMaleMice, Inbred C57BLMicroRNAsOxygenRetinaRetinal NeovascularizationRetinopathy of PrematurityMicroRNAsOxygen

Identifiers

PMID27922698
PMCPMC5355681
OpenAlexW2560163622

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.