Evidence map›Paper›PMID 27974147›Full record

ArticleBiochemical pharmacology2017

Dual activities of ritanserin and R59022 as DGKα inhibitors and serotonin receptor antagonists.

Salome Boroda, Maria Niccum, Vidisha Raje, Benjamin W Purow, Thurl E Harris

Open access · greenAbstract read
In one paragraph

Article in Biochemical pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 66 citations in OpenAlex.

  1. Article
  2. bioRxiv : the preprint server for biology · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Diacylglycerol kinase is a keystone regulator of signaling relevant to the pathophysiology of asthma.American journal of physiology. Lung cellular and molecular physiology · 2024
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Salome BorodaDepartment of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
Maria NiccumDepartment of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
Vidisha RajeDepartment of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
Benjamin W PurowDepartment of Neurology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA. Electronic address: bwp5g@virginia.edu.
Thurl E HarrisDepartment of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA. Electronic address: teh3c@virginia.edu.
University of Virginia · US

Funding

TRAINING IN THE PHARMACOLOGICAL SCIENCEST32GM007055 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI LYNCH, KEVIN R. · 1985 to 2022
$7.2M
Novel DGKalpha inhibitors and immunotherapy for GBM and melanoma brain metastasisR01CA189524 · NCI · UNIVERSITY OF VIRGINIA · PI PUROW, BENJAMIN W. · 2014 to 2018
$2.5M
Role of Oxidized neutral lipids in adipocyte functionR01DK101946 · NIDDK · UNIVERSITY OF VIRGINIA · PI HARRIS, THURL E. · 2014 to 2018
$1.7M
Targeting diacylglycerol kinases in glioblastomaR01CA180699 · NCI · UNIVERSITY OF VIRGINIA · PI PUROW, BENJAMIN W. · 2014 to 2017
$1.6M
NCI NIH HHS R01 CA180699NCI NIH HHS R01 CA189524NIDDK NIH HHS R01 DK101946NIGMS NIH HHS T32 GM007055
6 · The paper itself

Abstract

Diacylglycerol kinase alpha (DGKα) catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). Recently, DGKα was identified as a therapeutic target in various cancers, as well as in immunotherapy. Application of small-molecule DGK inhibitors, R59022 and R59949, induces cancer cell death in vitro and in vivo. The pharmacokinetics of these compounds in mice, however, are poor. Thus, there is a need to discover additional DGK inhibitors not only to validate these enzymes as targets in oncology, but also to achieve a better understanding of their biology. In the present study, we investigate the activity of ritanserin, a compound structurally similar to R59022, against DGKα. Ritanserin, originally characterized as a serotonin (5-HT) receptor (5-HTR) antagonist, underwent clinical trials as a potential medicine for the treatment of schizophrenia and substance dependence. We document herein that ritanserin attenuates DGKα kinase activity while increasing the enzyme's affinity for ATP in vitro. In addition, R59022 and ritanserin function as DGKα inhibitors in cultured cells and activate protein kinase C (PKC). While recognizing that ritanserin attenuates DGK activity, we also find that R59022 and R59949 are 5-HTR antagonists. In conclusion, ritanserin, R59022 and R59949 are combined pharmacological inhibitors of DGKα and 5-HTRs in vitro.

Indexed as

Adenosine TriphosphateDiacylglycerol KinaseElectrophoresis, Polyacrylamide GelEnzyme InhibitorsHEK293 CellsHeLa CellsHumansKineticsPyrimidinonesRitanserinThiazolesAdenosine TriphosphateDiacylglycerol KinaseEnzyme InhibitorsPyrimidinonesR 59022RitanserinThiazolesDiacylglycerol kinase (DGK)Diacylglycerol kinase inhibitor II (PubChem CID: 657356)Diacylglycerol kinase inhibitor I (PubChem CID: 3012)Ketanserin (PubChem CID: 3822).Protein kinase C (PKC)R59022RitanserinRitanserin (PubChem CID: 5074)Serotonin receptor (5-HTR)

Identifiers

PMID27974147
PMCPMC5164959
OpenAlexW2539781154

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.