ArticlePloS one2016
Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Re-establishment of TAD boundary organization during DNA replication.bioRxiv : the preprint server for biology · 2026Article
- Optical pooled screening for the discovery of regulators of the alternative lengthening of telomeres pathway.Methods (San Diego, Calif.) · 2025Article
- CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids.Cell and tissue research · 2025Review
- Genome-scale requirements for dynein-based transport revealed by a high-content arrayed CRISPR screen.The Journal of cell biology · 2024Article
- Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen.bioRxiv : the preprint server for biology · 2023Article
- A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs.The CRISPR journal · 2022Article
- Review
- Arrayed CRISPR Screening Identifies Novel Targets That Enhance the Productive Delivery of mRNA by MC3-Based Lipid Nanoparticles.SLAS discovery : advancing life sciences R & D · 2020Article
- CRISPR: A Screener's Guide.SLAS discovery : advancing life sciences R & D · 2020Article
- A solid-phase transfection platform for arrayed CRISPR screens.Molecular systems biology · 2019Article
- Chemogenetic interactions in human cancer cells.Computational and structural biotechnology journal · 2019Review
- Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection.Cell & bioscience · 2019Article
- Blank spots on the map: some current questions on nuclear organization and genome architecture.Histochemistry and cell biology · 2018Review
- Arrayed CRISPR screen with image-based assay reliably uncovers host genes required for coxsackievirus infection.Genome research · 2018Article
- CRISPR whole-genome screening identifies new necroptosis regulators and RIPK1 alternative splicing.Cell death & disease · 2018Article
- Am I ready for CRISPR? A user's guide to genetic screens.Nature reviews. Genetics · 2018Review
- Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens.Molecular systems biology · 2018Article
- Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition.PloS one · 2018Article
- High-Throughput Imaging for the Discovery of Cellular Mechanisms of Disease.Trends in genetics : TIG · 2017Review
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, lentiviral-based CRISPR-Cas9 screens have largely been conducted in pooled format. However, numerous assays are not amenable to pooled approaches, and lentiviral screening in arrayed format presents many challenges. We sought to examine synthetic CRISPR reagents in the context of arrayed screening. Experiments were performed using aberrant DNA replication as an assay. Using synthetic CRISPR RNAs targeting the known control gene GMNN in HCT-116 cells stably expressing Cas9, we observed statistically significant phenotype among the majority of transfected cells within 72 hours. Additional studies revealed near complete loss of GMNN protein and editing of GMNN DNA. We next conducted a screen of synthetic CRISPR RNAs directed against 640 ubiquitin-related genes. Screening identified known and novel DNA replication regulators that were also supported by siRNA gene knockdown. Notably, CRISPR screening identified more statistically significant hits than corresponding siRNA screens run in parallel. These results highlight the possibility of using synthetic CRISPR reagents as an arrayed screening tool.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.