ArticleMethods in enzymology2017
Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.
Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Oleate activates PLD2 lipase and GEF activity by modulating membrane microdomain dynamics via S-acylation.Journal of lipid research · 2025Article
- Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Expression of Phospholipase D Family Member 6 in Bovine Testes and Its Molecular Characteristics.International journal of molecular sciences · 2023Article
- Discovery of Phospholipase D Inhibitors with Improved Drug-like Properties and Central Nervous System Penetrance.ACS medicinal chemistry letters · 2022Article
- The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells.Cell communication and signaling : CCS · 2021Article
- IMPACT: Imaging phospholipase d activity with clickable alcohols via transphosphatidylation.Methods in enzymology · 2020Article
- A real-time, click chemistry imaging approach reveals stimulus-specific subcellular locations of phospholipase D activity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Identification of vaccine candidate proteins in Ureaplasma urealyticum causing infertility.Indian journal of sexually transmitted diseases and AIDSArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The phospholipase D (PLD) enzymatic superfamily regulates a wide range of cell biological and physiological pathways, including platelet activation, immune responses, cancer, and spermatogenesis. The three main enzymatic actions of the superfamily entail (i) hydrolyzing membrane phospholipids (phosphatidylcholine (PC) and cardiolipin) to generate choline and the second messenger signaling lipid phosphatidic acid (PA), (ii) using ethanol to transphosphatidylate PC to generate the long-lived metabolite phosphatidylethanol, and (iii) hydrolyzing RNA transcripts to generate piRNAs, the third form of endogenous RNAi. We discuss briefly previously published methods for in vitro and in vivo detection and imaging of PA, and focus on production, purification, and in vitro endonuclease activity analysis for human PLD6, a mitochondrial-tethered isoform with roles in fertility, cancer, and neuronal homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.