Evidence map›Paper›PMID 28076348›Full record

ArticlePLoS genetics2017

Reverse Pathway Genetic Approach Identifies Epistasis in Autism Spectrum Disorders.

Ileena Mitra, Alinoë Lavillaureix, Erika Yeh, Michela Traglia, Kathryn Tsang, Carrie E Bearden, Katherine A Rauen, Lauren A Weiss

Open access · goldAbstract read
In one paragraph

Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 46 citations in OpenAlex.

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  8. BiallelicSmall GTPases · 2022
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  14. The Prevalence of Insomnia and the Link between Iron Metabolism Genes Polymorphisms,International journal of environmental research and public health · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Ileena MitraDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.ORCID http://orcid.org/0000-0002-9882-3165
Alinoë LavillaureixDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.ORCID http://orcid.org/0000-0002-3727-9530
Erika YehDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.ORCID http://orcid.org/0000-0002-2374-1528
Michela TragliaDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.
Kathryn TsangDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.
Carrie E BeardenDepartment of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, Los Angeles, California, United States of America.
Katherine A RauenInstitute for Human Genetics, University of California San Francisco, San Francisco, California, United States of America.
Lauren A WeissDepartment of Psychiatry, University of California San Francisco, San Francisco, California, United States of America.ORCID http://orcid.org/0000-0002-5700-135X
University of California, San Francisco · USDélégation Paris 5 · FRUniversity of California, Los Angeles · US

Funding

Dissecting Epistasis and Pleiotropy in Autism towards Personalized MedicineDP2OD007449 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WEISS, LAUREN ANNE · 2010 to 2014
$2.4M
Neurofibromatosis Type I as a Model for Therapeutic NeuroadaptationR34MH089299 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BEARDEN, CARRIE E · 2009 to 2011
$691k
NIH HHS DP2 OD007449NIMH NIH HHS R34 MH089299
6 · The paper itself

Abstract

Although gene-gene interaction, or epistasis, plays a large role in complex traits in model organisms, genome-wide by genome-wide searches for two-way interaction have limited power in human studies. We thus used knowledge of a biological pathway in order to identify a contribution of epistasis to autism spectrum disorders (ASDs) in humans, a reverse-pathway genetic approach. Based on previous observation of increased ASD symptoms in Mendelian disorders of the Ras/MAPK pathway (RASopathies), we showed that common SNPs in RASopathy genes show enrichment for association signal in GWAS (P = 0.02). We then screened genome-wide for interactors with RASopathy gene SNPs and showed strong enrichment in ASD-affected individuals (P < 2.2 x 10-16), with a number of pairwise interactions meeting genome-wide criteria for significance. Finally, we utilized quantitative measures of ASD symptoms in RASopathy-affected individuals to perform modifier mapping via GWAS. One top region overlapped between these independent approaches, and we showed dysregulation of a gene in this region, GPR141, in a RASopathy neural cell line. We thus used orthogonal approaches to provide strong evidence for a contribution of epistasis to ASDs, confirm a role for the Ras/MAPK pathway in idiopathic ASDs, and to identify a convergent candidate gene that may interact with the Ras/MAPK pathway.

Indexed as

Epistasis, GeneticAutism Spectrum DisorderCell LineFemaleGenes, ModifierGenome-Wide Association StudyHumansMaleMAP Kinase Signaling SystemNeural Stem CellsPolymorphism, Single Nucleotideras ProteinsReceptors, G-Protein-Coupledras ProteinsReceptors, G-Protein-Coupled

Identifiers

PMID28076348
PMCPMC5226683
OpenAlexW2572835674

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.