ArticleNature communications2017
Infrared laser-induced gene expression for tracking development and function of single C. elegans embryonic neurons.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Heat-mediated manipulation of gene expression by IR-LEGO in the developing genitalia in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Embryonic Development of Caenorhabditis elegans Sense Organs.The Journal of comparative neurology · 2025Article
- Glia Development and Function in the NematodeCold Spring Harbor perspectives in biology · 2024Review
- Article
- A developmental pathway for epithelial-to-motoneuron transformation in C. elegans.Cell reports · 2022Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Patched-Related Is Required for Proper Development of EmbryonicFrontiers in neuroscience · 2022Article
- DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans.PLoS genetics · 2021Article
- Caenorhabditis elegans PTR/PTCHD PTR-18 promotes the clearance of extracellular hedgehog-related protein via endocytosis.PLoS genetics · 2021Article
- Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans.Scientific reports · 2021Article
- Review
- Article
- Review
- Probing and manipulating embryogenesis via nanoscale thermometry and temperature control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Novel Technological Advances in Functional Connectomics inJournal of developmental biology · 2019Review
- Cell type-specific structural plasticity of the ciliary transition zone in C. elegans.Biology of the cell · 2019Article
- FLIRT: fast local infrared thermogenetics for subcellular control of protein function.Nature methods · 2018Article
- Ordered arrangement of dendrites within aeLife · 2018Article
- Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal transgene control inProceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Visualizing neural-circuit assembly in vivo requires tracking growth of optically resolvable neurites. The Caenorhabditis elegans embryonic nervous system, comprising 222 neurons and 56 glia, is attractive for comprehensive studies of development; however, embryonic reporters are broadly expressed, making single-neurite tracking/manipulation challenging. We present a method, using an infrared laser, for reproducible heat-dependent gene expression in small sublineages (one to four cells) without radiation damage. We go beyond proof-of-principle, and use our system to label and track single neurons during early nervous-system assembly. We uncover a retrograde extension mechanism for axon growth, and reveal the aetiology of axon-guidance defects in sax-3/Robo and vab-1/EphR mutants. We also perform cell-specific rescues, determining DAF-6/patched-related site of action during sensory-organ development. Simultaneous ablation and labelling of cells using our system reveals roles for glia in dendrite extension. Our method can be applied to other optically/IR-transparent organisms, and opens the door to high-resolution systematic analyses of C. elegans morphogenesis.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.