Evidence mapPaperPMID 28099155Full record

ArticleOncotarget2017

Metformin promotes apoptosis in hepatocellular carcinoma through the CEBPD-induced autophagy pathway.

Hsin-Hwa Tsai, Hong-Yue Lai, Yueh-Chiu Chen, Chien-Feng Li, Huei-Sheng Huang, Hsiao-Sheng Liu, Yau-Sheng Tsai, Ju-Ming Wang

Open access · diamondAbstract read
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 69 citations in OpenAlex.

  1. Article
  2. Review
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  7. Review
  8. Article
  9. Article
  10. Role of metformin in the diagnosis, prevention, and treatment of hepatocellular carcinoma.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2022
    Article
  11. Review
  12. Single-cell RNA profiling ofFrontiers in cellular and infection microbiology · 2022
    Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Hsin-Hwa TsaiInstitute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan, Taiwan.
Hong-Yue LaiInstitute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.
Yueh-Chiu ChenDepartment of Pharmacology, National Cheng Kung University, Tainan, Taiwan.
Chien-Feng LiDepartment of Pathology, Chi-Mei Medical Center, Tainan, Taiwan.
Huei-Sheng HuangDepartment of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
Hsiao-Sheng LiuDepartment of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan.
Yau-Sheng TsaiInstitute of Clinical Medicine, National Cheng Kung University, Tainan, Taiwan.
Ju-Ming WangInstitute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan, Taiwan.
National Cheng Kung University · TWChi Mei Medical Center · TWTaipei Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Metformin, as an AMP-activated protein kinase (AMPK) activator, can activate autophagy. A study showed that metformin decreased the risk of hepatocellular carcinoma (HCC) in diabetic patients. However, the detailed mechanism in the metformin-mediated anticancer effect remains an open question. Transcription factor CCAAT/enhancer-binding protein delta (CEBPD) has been suggested to serve as a tumor suppressor and is responsive to multiple anticancer drugs in HCC. In this study, we found that CEBPD and autophagy are involved in metformin-induced cell apoptosis in Huh7 cells. The underlying mechanisms in this process included a reduction in Src-mediated CEBPD protein degradation and an increase in CEBPD-regulated LC3B and ATG3 gene transcription under metformin treatment. We also found that AMPK is involved in metformin-induced CEBPD expression. Combined treatment with metformin and rapamycin can enhance autophagic cell death through the AMPK-dependent and AMPK-independent pathway, respectively. Taken together, we provide a new insight and therapeutic approach by targeting autophagy in the treatment of HCC.

Indexed as

AnimalsAntineoplastic AgentsApoptosisAutophagyBlotting, WesternCarcinoma, HepatocellularCCAAT-Enhancer-Binding Protein-deltaCell Line, TumorChromatin ImmunoprecipitationFlow CytometryGene Knockdown TechniquesHeterograftsHumansHypoglycemic AgentsLiver NeoplasmsMaleAntineoplastic AgentsCCAAT-Enhancer-Binding Protein-deltaCEBPD protein, humanHypoglycemic AgentsMetforminapoptosisautophagyCEBPDHCCmetformin

Identifiers

PMID28099155
PMCPMC5355142
OpenAlexW2576839195

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.