ArticlePloS one2017
Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The chitin receptor-interacting protein LIK1 regulates extracellular ATP signaling via interaction with P2K1 inbioRxiv : the preprint server for biology · 2026Article
- Distinct domains of ENHANCER OF PINOID hold information for its polarization required for auxin-mediated cotyledon and flower development in Arabidopsis.PLoS genetics · 2025Article
- Guidelines for naming and studying plasma membrane domains in plants.Nature plants · 2024Review
- Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis.Nature communications · 2024Article
- Article
- It takes two to tango - molecular links between plant immunity and brassinosteroid signalling.Journal of cell science · 2020Review
- State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks.International journal of molecular sciences · 2020Review
- The manifold actions of signaling peptides on subcellular dynamics of a receptor specify stomatal cell fate.eLife · 2020Article
- Regulation of Three Key Kinases of Brassinosteroid Signaling Pathway.International journal of molecular sciences · 2020Review
- The BIR2/BIR3-Associated Phospholipase Dγ1 Negatively Regulates Plant Immunity.Plant physiology · 2020Article
- The Nanoscale Organization of the Plasma Membrane and Its Importance in Signaling: A Proteolipid Perspective.Plant physiology · 2020Review
- Defining the dance: quantification and classification of endoplasmic reticulum dynamics.Journal of experimental botany · 2020Article
- Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function.International journal of molecular sciences · 2020Review
- The cell wall regulates dynamics and size of plasma-membrane nanodomains inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance.International journal of molecular sciences · 2018Review
- Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells.Journal of experimental botany · 2018Article
- Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase.Journal of lipid research · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassinosteroids (BRs) are plant hormones that are perceived at the plasma membrane (PM) by the ligand binding receptor BRASSINOSTEROID-INSENSITIVE1 (BRI1) and the co-receptor SOMATIC EMBRYOGENESIS RECEPTOR LIKE KINASE 3/BRI1 ASSOCIATED KINASE 1 (SERK3/BAK1). To visualize BRI1-GFP and SERK3/BAK1-mCherry in the plane of the PM, variable-angle epifluorescence microscopy (VAEM) was employed, which allows selective illumination of a thin surface layer. VAEM revealed an inhomogeneous distribution of BRI1-GFP and SERK3/BAK1-mCherry at the PM, which we attribute to the presence of distinct nanoclusters. Neither the BRI1 nor the SERK3/BAK1 nanocluster density is affected by depletion of endogenous ligands or application of exogenous ligands. To reveal interacting populations of receptor complexes, we utilized selective-surface observation-fluorescence lifetime imaging microscopy (SSO-FLIM) for the detection of Förster resonance energy transfer (FRET). Using this approach, we observed hetero-oligomerisation of BRI1 and SERK3 in the nanoclusters, which did not change upon depletion of endogenous ligand or signal activation. Upon ligand application, however, the number of BRI1-SERK3 /BAK1 hetero-oligomers was reduced, possibly due to endocytosis of active signalling units of BRI1-SERK3/BAK1 residing in the PM. We propose that formation of nanoclusters in the plant PM is subjected to biophysical restraints, while the stoichiometry of receptors inside these nanoclusters is variable and important for signal transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.