ArticleFrontiers in plant science2017
Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 42 citations in OpenAlex.
- Integrated proteomic analysis reveals adaptation mechanisms of paper mulberry seedling leaves to nitrogen stress.BMC plant biology · 2026Article
- Nitrogen Topdressing Rate Alters Starch and Protein Properties in Grains at Different Spike Positions Under Long-Term Field Conditions.Plants (Basel, Switzerland) · 2025Article
- Post-transcriptional regulation dominates protein biosynthesis inFrontiers in plant science · 2025Article
- Biochar and Nitrogen Fertilizer Change the Quality of Waxy and Non-Waxy Broomcorn Millet (Foods (Basel, Switzerland) · 2023Article
- Genetic Dissection of Spike Productivity Traits in the Siberian Collection of Spring Barley.Biomolecules · 2023Article
- Structure and assembly process of fungal communities in the Yangtze River Estuary.Frontiers in microbiology · 2023Article
- The importance of conditionally rare taxa for the assembly and interaction of fungal communities in mangrove sediments.Applied microbiology and biotechnology · 2022Article
- Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice.International journal of molecular sciences · 2021Review
- New insights into the mechanism of storage protein biosynthesis in wheat caryopsis under different nitrogen levels.Protoplasma · 2020Article
- Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Effects of Independent and Combined Water-Deficit and High-Nitrogen Treatments on Flag Leaf Proteomes during Wheat Grain Development.International journal of molecular sciences · 2020Article
- Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development.Botanical studies · 2018Article
- Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms.Journal of experimental botany · 2018Article
- Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nitrogen (N) is a macronutrient important for plant growth and development. It also strongly influences starch and protein synthesis, closely related to grain yield and quality. We performed the first comparative phosphoproteomic analysis of developing wheat grains in response to high-N fertilizer. Physiological and biochemical analyses showed that application of high-N fertilizer resulted in significant increases in leaf length and area, chlorophyll content, the activity of key enzymes in leaves such as nitrate reductase (NR), and in grains such as sucrose phosphate synthase (SPS), sucrose synthase (SuSy), and ADP glucose pyrophosphorylase (AGPase). This enhanced enzyme activity led to significant improvements in starch content, grain yield, and ultimately, bread making quality. Comparative phosphoproteomic analysis of developing grains under the application of high-N fertilizer performed 15 and 25 days post-anthesis identified 2470 phosphosites among 1372 phosphoproteins, of which 411 unique proteins displayed significant changes in phosphorylation level (>2-fold or <0.5-fold). These phosphoproteins are involved mainly in signaling transduction, starch synthesis, energy metabolism. Pro-Q diamond staining and Western blotting confirmed our phosphoproteomic results. We propose a putative pathway to elucidate the important roles of the central phosphoproteins regulating grain starch and protein synthesis. Our results provide new insights into the molecular mechanisms of protein phosphorylation modifications involved in grain development, yield and quality formation.
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