ArticleScientific reports2017
Deep sequencing and transcriptome analyses to identify genes involved in secoiridoid biosynthesis in the Tibetan medicinal plant Swertia mussotii.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 65 citations in OpenAlex.
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- Regulation of gene expression in the secondary metabolic synthesis pathway ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Integrative metabolomic and transcriptomic analyses reveals the accumulation patterns of key metabolites associated with flavonoids and terpenoids of Gynostemma pentaphyllum (Thunb.) Makino.Scientific reports · 2024Article
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- Transcriptome analysis and exploration of genes involved in the biosynthesis of secoiridoids inPeerJ · 2023Article
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- Global gene expression profiling under nitrogen stress identifies key genes involved in nitrogen stress adaptation in maize (Zea mays L.).Scientific reports · 2022Article
- Application of High-Throughput Sequencing on the Chinese Herbal Medicine for the Data-Mining of the Bioactive Compounds.Frontiers in plant science · 2022Review
- Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss.Scientific reports · 2021Article
- Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis.Scientific reports · 2021Article
- Biotechnological interventions and genetic diversity assessment in Swertia sp.: a myriad source of valuable secondary metabolites.Applied microbiology and biotechnology · 2021Review
- Full-Length Transcriptome Sequencing Provides Insights into Flavonoid Biosynthesis inLife (Basel, Switzerland) · 2021Article
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- Transcriptomics of the Rooibos (Biotech (Basel (Switzerland)) · 2020Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Swertia mussotii Franch. is an important traditional Tibetan medicinal plant with pharmacological properties effective in the treatment of various ailments including hepatitis. Secoiridoids are the major bioactive compounds in S. mussotii. To better understand the secoiridoid biosynthesis pathway, we generated transcriptome sequences from the root, leaf, stem, and flower tissues, and performed de novo sequence assembly, yielding 98,613 unique transcripts with an N50 of 1,085 bp. Putative functions could be assigned to 35,029 transcripts (35.52%) based on BLAST searches against annotation databases including GO and KEGG. The expression profiles of 39 candidate transcripts encoding the key enzymes for secoiridoid biosynthesis were examined in different S. mussotii tissues, validated by qRT-PCR, and compared with the homologous genes from S. japonica, a species in the same family, unveiling the gene expression, regulation, and conservation of the pathway. The examination of the accumulated levels of three bioactive compounds, sweroside, swertiamarin, and gentiopicroside, revealed their considerable variations in different tissues, with no significant correlation with the expression profiles of key genes in the pathway, suggesting complex biological behaviours in the coordination of metabolite biosynthesis and accumulation. The genomic dataset and analyses presented here lay the foundation for further research on this important medicinal plant.
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