ArticleScientific reports2017
A reliable and flexible gene manipulation strategy in posthatch zebra finch brain.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- AAV-mediated transduction of songbird retina.Frontiers in physiology · 2025Article
- Long-term, high-resolution in vivo calcium imaging in pigeons.Cell reports methods · 2024Article
- Genomic Approaches Are Improving Taxonomic Representation in Genetic Studies of Speciation.Cold Spring Harbor perspectives in biology · 2024Review
- A custom-made AAV1 variant (AAV1-T593K) enables efficient transduction of Japanese quail neurons in vitro and in vivo.Communications biology · 2023Article
- Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation.BMC biotechnology · 2022Article
- Generation and characterization of genome-modified chondrocyte-like cells from the zebra finch cell line immortalized by c-MYC expression.Frontiers in zoology · 2022Article
- Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.Scientific reports · 2022Article
- A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience.Frontiers in molecular neuroscience · 2022Review
- Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.Scientific reports · 2021Article
- Article
- AAV1 is the optimal viral vector for optogenetic experiments in pigeons (Columba livia).Communications biology · 2021Article
- Gene manipulation to test links between genome, brain and behavior in developing songbirds: a test case.The Journal of experimental biology · 2020Review
- Development of an Acute Method to Deliver Transgenes Into the Brains of AdultFrontiers in neural circuits · 2018Article
- Unraveling of Central Nervous System Disease Mechanisms Using CRISPR Genome Manipulation.Journal of central nervous system disease · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Songbird models meaningfully contribute to many fields including learned vocal communication, the neurobiology of social interactions, brain development, and ecology. The value of investigating gene-brain-behavior relationships in songbirds is therefore high. Viral infections typically used in other lab animals to deliver gene editing constructs have been less effective in songbirds, likely due to immune system properties. We therefore leveraged the in vivo electroporation strategy used in utero in rodents and in ovo in poultry, and apply it to posthatch zebra finch songbird chicks. We present a series of experiments with a combination of promoters, fluorescent protein genes, and piggyBac transposase vectors to demonstrate that this can be a reliable, efficient, and flexible strategy for genome manipulation. We discuss options for gene delivery experiments to test circuit and behavioral hypotheses using a variety of manipulations, including gene overexpression, CRISPR/Cas9 gene editing, inducible technologies, optogenetic or DREADD cellular control, and cell type-specific expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.