ArticleTheriogenology2017
An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs.
Article in Theriogenology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Cryogenic Preservation of Xenopus Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optimizing Sperm Cryopreservation from Four Endangered Korean Amphibian Species: Species-Specific Effects of Cryoprotectants and Cooling Regimes on Membrane-Integrity Viability.Animals : an open access journal from MDPI · 2025Article
- Intracytoplasmic Sperm Injection Using 20-Year-Old Cryopreserved Sperm Results in Normal, Viable, and Reproductive Offspring inAnimals : an open access journal from MDPI · 2025Article
- Transitioning from a research protocol to a scalable applied pathway for Xenopus laevis sperm cryopreservation at a national stock center: The effect of cryoprotectants.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- The Increasing Role of Short-Term Sperm Storage and Cryopreservation in Conserving Threatened Amphibian Species.Animals : an open access journal from MDPI · 2023Review
- Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology.Animal reproduction · 2023Review
- Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny.Animals : an open access journal from MDPI · 2022Article
- Conservation Biology and Reproduction in a Time of Developmental Plasticity.Biomolecules · 2022Review
- Biallelic variants in COPB1 cause a novel, severe intellectual disability syndrome with cataracts and variable microcephaly.Genome medicine · 2021Article
- Opposite Modulation of RAC1 by Mutations in TRIO Is Associated with Distinct, Domain-Specific Neurodevelopmental Disorders.American journal of human genetics · 2020Article
- Workshop report: Cryopreservation of aquatic biomedical models.Cryobiology · 2019Article
- Discussing the future of amphibians in research.Lab animal · 2019Article
- Review
- Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Generation of a sexually mature individual of the Eastern dwarf tree frog,Conservation physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Cryogenic storage of sperm from genetically altered Xenopus improves cost effectiveness and animal welfare associated with their use in research; currently it is routine for X. tropicalis but not reliable for X. laevis. Here we compare directly the three published protocols for Xenopus sperm freeze-thaw and determine whether sperm storage temperature, method of testes maceration and delays in the freezing protocols affect successful fertilisation and embryo development in X. laevis. We conclude that the protocol is robust and that the variability observed in fertilisation rates is due to differences between individuals. We show that the embryos made from the frozen-thawed sperm are normal and that the adults they develop into are reproductively indistinguishable from others in the colony. This opens the way for using cryopreserved sperm to distribute dominant genetically altered (GA) lines, potentially saving travel-induced stress to the male frogs, reducing their numbers used and making Xenopus experiments more cost effective.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.