ArticleJournal of leukocyte biology2017
Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.
Article in Journal of leukocyte biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed.
- Dissociation method influences immune cell composition in porcine tonsil cell suspensions.Frontiers in immunology · 2026Article
- Dendritic cells and HIV transmission: roles and subsets of antigen-presenting cells in the human anogenital tract.PLoS pathogens · 2025Review
- Early responses of primary human and bovine monocytes, monocytic THP-1 cells and THP-1 cell-derived macrophages to vitalFrontiers in immunology · 2025Article
- Characterising plasmacytoid and myeloid AXL+ SIGLEC-6+ dendritic cell functions and their interactions with HIV.PLoS pathogens · 2024Article
- OMIP-096: A 24-color flow cytometry panel to identify and characterize CD4+ and CD8+ tissue-resident T cells in human skin, intestinal, and type II mucosal tissue.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- Dynamic thresholding and tissue dissociation optimization for CITE-seq identifies differential surface protein abundance in metastatic melanoma.Communications biology · 2023Article
- Enhancing the immunogenicity of cancer vaccines by harnessing CLEC9A.Human vaccines & immunotherapeutics · 2022Article
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- Differential expansion of T peripheral helper cells in early rheumatoid arthritis and osteoarthritis synovium.RMD open · 2022Article
- Revisiting the Experimental Methods for Human Skin T-Cell Analysis.JID innovations : skin science from molecules to population health · 2022Article
- HIV transmitting mononuclear phagocytes; integrating the old and new.Mucosal immunology · 2022Review
- Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain.Nature neuroscience · 2022Article
- An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies.Frontiers in cell and developmental biology · 2022Article
- Article
- Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells.Biomedicines · 2021Article
- Changes in Immune Cell Types with Age in Breast are Consistent with a Decline in Immune Surveillance and Increased Immunosuppression.Journal of mammary gland biology and neoplasia · 2021Article
- Making the most of high-dimensional cytometry data.Immunology and cell biology · 2021Review
- Targeting human langerin promotes HIV-1 specific humoral immune responses.PLoS pathogens · 2021Article
- Human anogenital monocyte-derived dendritic cells and langerin+cDC2 are major HIV target cells.Nature communications · 2021Article
- Herpes Simplex Virus type 1 infects Langerhans cells and the novel epidermal dendritic cell, Epi-cDC2s, via different entry pathways.PLoS pathogens · 2021Article
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
Mononuclear phagocytes are present in skin and mucosa and represent one of the first lines of defense against invading pathogens, which they detect via an array of pathogen-binding receptors expressed on their surface. However, their extraction from tissue is difficult, and the isolation technique used has functional consequences on the cells obtained. Here, we compare mononuclear phagocytes isolated from human skin using either enzymatic digestion or spontaneous migration. Cells isolated via enzymatic digestion are in an immature state, and all subsets are easily defined. However, cells isolated by spontaneous migration are in a mature state, and CD141 cross-presenting DCs (cDC1) are more difficult to define. Different pathogen-binding receptors are susceptible to cleavage by blends of collagenase, demonstrating that great care must be taken in choosing the correct enzyme blend to digest tissue if carrying out pathogen-interaction assays. Finally, we have optimized mononuclear phagocyte culture conditions to enhance their survival after liberation from the tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.