Evidence map›Paper›PMID 28369094›Full record

ArticlePloS one2017

Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification.

Shimeng Fang, Hongzhu Tian, Xiancheng Li, Dong Jin, Xiaojie Li, Jing Kong, Chun Yang, Xuesong Yang, Yao Lu, Yong Luo and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
120citing papers in PubMed, 1 pooled it
9.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

120 citing papers in PubMed, 1 synthesis or guideline pooled it, 222 citations in OpenAlex.

  1. Effect of exosome biomarkers for diagnosis and prognosis of breast cancer patients.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2018
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60 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Shimeng FangCollege of Stomatology, Dalian Medical University, Dalian, China.
Hongzhu TianCollege of Stomatology, Dalian Medical University, Dalian, China.
Xiancheng LiDepartment of Urology, the Second Affiliated Hospital, Dalian Medical University, Dalian, China.
Dong JinCollege of Stomatology, Dalian Medical University, Dalian, China.
Xiaojie LiCollege of Stomatology, Dalian Medical University, Dalian, China.
Jing KongCollege of Stomatology, Dalian Medical University, Dalian, China.
Chun YangDepartment of Nuclear Medicine, the First Affiliated Hospital, Dalian Medical University, Dalian, China.
Xuesong YangDepartment of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, China.
Yao LuDepartment of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Yong LuoState Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.
Bingcheng LinDepartment of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Weidong NiuCollege of Stomatology, Dalian Medical University, Dalian, China.
Tingjiao LiuCollege of Stomatology, Dalian Medical University, Dalian, China.ORCID http://orcid.org/0000-0001-5850-789X
Dalian Medical University · CNDalian Institute of Chemical Physics · CNDalian University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes overexpress a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.

Indexed as

Biomarkers, TumorBreast NeoplasmsCell Line, TumorEpithelial Cell Adhesion MoleculeErb-b2 Receptor Tyrosine KinasesExosomesFemaleHumansLab-On-A-Chip DevicesMCF-7 CellsMicrofluidic Analytical TechniquesMicroscopy, Electron, TransmissionNeoplastic Cells, CirculatingBiomarkers, TumorEPCAM protein, humanEpithelial Cell Adhesion MoleculeERBB2 protein, humanErb-b2 Receptor Tyrosine Kinases

Identifiers

PMID28369094
PMCPMC5378374
OpenAlexW2604622315

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.