Evidence map›Paper›PMID 28374173›Full record

ArticleThe protein journal2017

Structural Stability of Streptococcal Serum Opacity Factor.

Dedipya Yelamanchili, Baiba K Gillard, Antonio M Gotto, Henry J Pownall, Corina Rosales

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The protein journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dedipya YelamanchiliThe Center for Bioenergetics and Metabolism, Houston Methodist Research Institute, 6670 Bertner St., Houston, TX, 77030, USA.
Baiba K GillardThe Center for Bioenergetics and Metabolism, Houston Methodist Research Institute, 6670 Bertner St., Houston, TX, 77030, USA.
Antonio M GottoThe Center for Bioenergetics and Metabolism, Houston Methodist Research Institute, 6670 Bertner St., Houston, TX, 77030, USA.
Henry J PownallThe Center for Bioenergetics and Metabolism, Houston Methodist Research Institute, 6670 Bertner St., Houston, TX, 77030, USA.
Corina RosalesThe Center for Bioenergetics and Metabolism, Houston Methodist Research Institute, 6670 Bertner St., Houston, TX, 77030, USA. crosales@houstonmethodist.org.ORCID 0000-0002-9068-2775
Houston Methodist · US

Funding

Therapeutic Approaches to Dysregulated HDL MetabolismR01HL056865 · NHLBI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI POWNALL, HENRY J. · 1997 to 2015
$5.2M
NHLBI NIH HHS R01 HL056865
6 · The paper itself

Abstract

Streptococcal serum opacity factor (SOF) is a protein that clouds the plasma of multiple mammalian species by disrupting high density lipoprotein (HDL) structure. Intravenous infusion of low dose SOF (4 µg) into mice reduces their plasma cholesterol concentrations ~ 40% in 3 h. Here we investigated the effects of pH, ionic strength, temperature, and denaturation with guanidinium chloride (GdmCl) on SOF stability and its reaction vs HDL. SOF stability was tested by pre-incubation of SOF at various temperatures, pH's, and GdmCl concentrations and measuring the SOF reaction rate at pH 7.4 and 37 °C. SOF retained activity at temperatures up to 58 °C, at pH 4 to 10, and in 8.5 M GdmCl after being returned to standard buffer conditions. The effects of GdmCl, pH, and ionic strength on the SOF reaction rates were also measured. SOF was inactive at GdmCl ≥ 1 M; SOF was most active at pH 5, near its isoelectric point and at an ionic strength of 3 (in NaCl). These data reveal that SOF is a stable protein and suggest that its activity is determined, in part, by the effects of pH and ionic strength on its overall charge relative to that of its reaction target, HDL.

Indexed as

Bacterial ProteinsEnzyme StabilityHot TemperatureHydrogen-Ion ConcentrationOsmolar ConcentrationPeptide HydrolasesProtein DomainsStreptococcusBacterial Proteinsopacity factorPeptide HydrolasesCholesterolHigh density lipoproteinsProtein stabilitySerum opacity factor

Identifiers

PMID28374173
PMCPMC5448415
OpenAlexW2605340877

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.