ArticleBiomedical chromatography : BMC2017
A novel mass spectrometry-based method for simultaneous determination of asymmetric and symmetric dimethylarginine, l-arginine and l-citrulline optimized for LC-MS-TOF and LC-MS/MS.
Article in Biomedical chromatography : BMC, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It reports registered trial NCT03398356. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Assessment of the Effect of Metformin and Its Serum Concentration on the Concentration of Substances Associated With the Production of Nitric Oxide in Patients With Impaired Carbohydrate Metabolism
Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Analysis of catabolic products of L-arginine; L-ornithine and L-citrulline and the residual L-arginine using the HPLC and LC-MS.PloS one · 2026Article
- Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes.International journal of molecular sciences · 2024Article
- Nitric Oxide Synthesis Metabolites-As Potential Markers in Chronic Kidney Disease in Children.Current issues in molecular biology · 2022Article
- Low metformin dose and its therapeutic serum concentration in prediabetes.Scientific reports · 2021 · on this mapArticle
- Developing a robust, fast and reliable measurement method for the analysis of methylarginine derivatives and related metabolites.Journal of mass spectrometry and advances in the clinical lab · 2021Article
- LC-MS analysis ofRSC advances · 2020Article
- Metabolites of the Nitric Oxide (NO) Pathway Are Altered and Indicative of Reduced NO and Arginine Bioavailability in Patients with Cardiometabolic Diseases Complicated with Chronic Wounds of Lower Extremities: Targeted Metabolomics Approach (LC-MS/MS).Oxidative medicine and cellular longevity · 2019Article
- Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM.Cell death & disease · 2018Article
- High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry.Plant methods · 2018Article
- Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography-Mass Spectrometric Method.Chromatographia · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) is a regulatory molecule involved in many biological processes. NO is produced by nitric oxide synthase by conversion of l-arginine to l-citrulline. l-Arginine methylated derivatives, asymmetric and symmetric dimethylarginines (asymmetric dimethylarginine, ADMA, and symmetric dimethylarginine, SDMA), regulate l-arginine availability and the activity of nitric oxide synthase. As such, they have been frequently investigated as potential biomarkers in pathologies associated with dysfunctions in NO synthesis. Here, we present a new multistep analytical methodology based on liquid chromatography combined with mass spectrometry for the accurate identification of l-arginine, l-citrulline, ADMA and SDMA. Compounds are measured as stable 2,3,4,5,6-pentafluorobenzoyl chloride derivatives, which allows for simultaneous analysis of all compounds through chromatographic separation of ADMA and SDMA using a reverse-phase column. Serum aliquots (100 μL) were spiked with isotope-labeled internal standards and sodium carbonate buffer. The derivatization process was carried out at 25°C for 10 minu using pentafluorobenzoyl chloride as derivatization reagent. Calibration demonstrated good linearity (R
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.