Evidence mapPaperPMID 28447181Full record

ArticleCardiovascular drugs and therapy2017

SGLT-2 Inhibition with Dapagliflozin Reduces the Activation of the Nlrp3/ASC Inflammasome and Attenuates the Development of Diabetic Cardiomyopathy in Mice with Type 2 Diabetes. Further Augmentation of the Effects with Saxagliptin, a DPP4 Inhibitor.

Yumei Ye, Mandeep Bajaj, Hsiu-Chiung Yang, Jose R Perez-Polo, Yochai Birnbaum

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Cardiovascular drugs and therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05618223 (Dapagliflozin Effect on Rheumatic Mitral Stenosis), which is not on this map. Cited by 220 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
220citing papers in PubMed, 3 pooled it
16.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05618223 phase3unknown statusstarted 2022, after this paper: background citation

Dapagliflozin Effect on Rheumatic Mitral Stenosis

Ran2022Enrolled36Registered outcomes4Posted comparisons0ConditionsHeart Failure, Mitral Stenosis, Rheumatic Heart DiseaseArmsdapagliflozin
Open the trial in the graph
3 · Its place in the literature

Who cites it

220 citing papers in PubMed, 3 syntheses or guidelines pooled it, 361 citations in OpenAlex.

  1. Impact of DPP-4 Inhibitors on Interleukin Levels in Type 2 Diabetes Mellitus.The Journal of clinical endocrinology and metabolism · 2025
    Pooled it
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  5. Additive Effects of Quercetin-RichPharmaceuticals (Basel, Switzerland) · 2026
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  20. Guidelines for diet-induced models of cardiometabolic syndrome.American journal of physiology. Heart and circulatory physiology · 2025
    Review

160 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Yumei YeThe Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Mandeep BajajThe Section of Endocrinology, The Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
Hsiu-Chiung YangTranslational Science, CVMD, iMED, Astrazeneca, Mölndal, Sweden.
Jose R Perez-PoloThe Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Yochai BirnbaumThe Section of Cardiology, The Department of Medicine, Baylor College of Medicine, Houston, TX, 77030, USA. ybirnbau@bcm.edu.ORCID http://orcid.org/0000-0001-7653-6328
Baylor College of Medicine · USThe University of Texas Medical Branch at Galveston · USAstraZeneca (Sweden) · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWe assessed whether (1) dapagliflozin (Dapa, an SGLT2-inhibitor) attenuates the deterioration of heart function Nlrp3 and inflammasome activation in diabetic mice. (2) The effects can be augmented with saxagliptin (Saxa), a DDP4-inhibitor. (3) Dapa effect is possibly SGLT2-independent on cardiofibroblasts in vitro.

methodsType 2 diabetic (BTBR ob/ob) and wild-type (WT) mice received vehicle, Dapa, or Dapa+Saxa for 8 weeks. Glucose tolerance test and echocardiogram were performed. Cardiofibroblasts from WT and BTBR hearts were incubated with Dapa and exposed to LPS.

resultsLeft ventricular ejection fraction (LVEF) was 81 ± 1% in the WT and 53 ± 1% in the T2D-cont mice. Dapa and Dapa+Saxa improved LVEF to 68 ± 1 and 74.6 ± 1% in the BTBR mice (p < 0.001). The mRNA levels of NALP3, ASC, IL-1β, IL-6, caspase-1, and TNFα were significantly higher in the BTBR compared to the WT hearts; and Dapa and Dapa+Saxa significantly attenuated these levels. Likewise, protein levels of NLRP3, TNFα, and caspase-1 were higher in the BTBR compared to the WT hearts and Dapa, and to a greater extent Dapa+Saxa, attenuated the increase in the BTBR mice. Collagen-1 and collagen-3 mRNA levels significantly increased in the BTBR mice and these increases were attenuated by Dapa and Dapa+Saxa. P-AMPK/total-AMPK ratio was significantly lower in the BTBR mice than in the WT mice. Dapa and Dapa+Saxa equally increased the ratio in the BTBR mice. This in vitro study showed that NALP3, ASC, IL-1β, and caspase-1 mRNA levels were higher in the BTBR cardiofibroblasts and attenuated with Dapa. The effect was AMPK-dependent and SGLT1-independent.

conclusionsDapa attenuated the activation of the inflammasome, fibrosis, and deterioration of LVEF in BTBR mice. The anti-inflammatory, anti-fibrotic effects are likely SGLT2- and glucose-lowering-independent, as they were replicated in the in vitro model. The effects on remodeling were augmented when Saxa was added to Dapa. Yet, adding Saxa to Dapa did not result in a greater effect on myocardial fibrosis and collagen levels.

Indexed as

Sodium-Glucose Transporter 2 InhibitorsAdamantaneAMP-Activated Protein KinasesAnimalsApoptosisBenzhydryl CompoundsCARD Signaling Adaptor ProteinsCells, CulturedDiabetes Mellitus, Type 2Diabetic CardiomyopathiesDipeptidesDipeptidyl Peptidase 4Dipeptidyl-Peptidase IV InhibitorsDisease Models, AnimalDrug Therapy, CombinationFibroblastsAdamantaneAMP-Activated Protein KinasesBenzhydryl CompoundsCARD Signaling Adaptor ProteinsdapagliflozinDipeptidesDipeptidyl Peptidase 4Dipeptidyl-Peptidase IV InhibitorsDpp4 protein, mouseGlucosidesHypoglycemic AgentsInflammasomesInflammation MediatorsNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mousePycard protein, mousesaxagliptinSlc5a2 protein, mouseSodium-Glucose Transporter 2Sodium-Glucose Transporter 2 InhibitorsDiabetes mellitusDPP-4 inhibitorFibrosisInflammationSGLT-2 inhibitor

Identifiers

PMID28447181
OpenAlexW2608233061

What Socratic holds

Textmetadata
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.