Evidence mapPaperPMID 28487965Full record

ArticleInternational journal of molecular medicine2017

HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes.

Xiaohan Jin, Zhongwei Xu, Jin Cao, Rui Yan, Ruicheng Xu, Ruiqiong Ran, Yongqiang Ma, Wei Cai, Rong Fan, Yan Zhang and 2 more

Open access · hybridAbstract read
In one paragraph

Article in International journal of molecular medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Oxidative Stress in Ischemic Heart Disease.Oxidative medicine and cellular longevity · 2020
    Review
  9. Mechanism of protective effect of carnosol on pig intestinal epithelial cells.International journal of clinical and experimental pathology · 2020
    Article
  10. Review
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Xiaohan JinTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Zhongwei XuCentral Laboratory, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Jin CaoDepartment of Basic Medicine, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Rui YanCentral Laboratory, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Ruicheng XuDepartment of Basic Medicine, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Ruiqiong RanTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Yongqiang MaTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Wei CaiTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Rong FanCentral Laboratory, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Yan ZhangCentral Laboratory, Logistics University of the Chinese People's Armed Police Force, Tianjin 300309, P.R. China.
Xin ZhouTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Yuming LiTianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin 300162, P.R. China.
Tianjin University · CNChinese People's Armed Police ForceLogistics University of People's Armed Police Force · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heme oxygenase-1 (HO-1) is an inducible and cytoprotective enzyme that provides a defense against oxidant damage. The present study screened 137 HO-1/interacting proteins using a profound co-immunoprecipitation (Co-IP) coupled with proteomics, and profiled the global HO-1 interactome network, including oxidative phosphorylation, endoplasmic reticulum and transport vesicle functions. Among these molecules, we observed that a novel interactor, emopamil-binding protein (EBP), is closely related to the cholesterol metabolism process. This study demonstrated that cholesterol promotes excessive oxidative stress and alters the energy metabolism in cardiomyocytes, further triggering numerous cardiovascular diseases. We observed that cholesterol caused the overexpression of EBP and HO-1 by the activation of AKT and Nrf2/mTOR pathways. In addition, HO-1 and EBP performed a myocardial protective function. The overexpression of HO-1 alleviated the cholesterol-induced excessive oxidative stress status by inhibition of the carbohydrate metabolism. Notably, we also confirmed that the loss of partial HO-1 activity aggravated the oxidative damage and cardiac systolic function induced by a high-fat diet in HO-1 heterozygous (HO-1+/-) mice. These findings indicate that the HO-1/EBP interaction plays a protective role in alleviating the dysfunction of oxidative stress and cardiac systolic function induced by cholesterol stimulation.

Indexed as

Signal TransductionAnimalsCarbohydrate MetabolismCarrier ProteinsCell LineCholesterolHeme Oxygenase-1HypoxiaMaleMice, Inbred C57BLMyocytes, CardiacNF-E2-Related Factor 2Oxidative StressProtein Interaction MapsProto-Oncogene Proteins c-aktSteroid IsomerasesCarrier ProteinsCholesterolEbp protein, mouseHeme Oxygenase-1mTOR protein, mouseNfe2l2 protein, mouseNF-E2-Related Factor 2Proto-Oncogene Proteins c-aktSteroid IsomerasesTOR Serine-Threonine Kinases

Identifiers

PMID28487965
PMCPMC5428940
OpenAlexW2613376977

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.