ArticleNature2017
Conversion of adult endothelium to immunocompetent haematopoietic stem cells.
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Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
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126citing papers in PubMed
17.4field-weighted citation impact, top 1% of its field
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Who cites it
126 citing papers in PubMed, 226 citations in OpenAlex.
- Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow.eLife · 2026Article
- Mechanometabolism instructs hematopoietic stem cell specification.The Journal of experimental medicine · 2026Article
- Generation of thymus-reconstituting T cell progenitors from human pluripotent stem cells.Cell reports methods · 2026Article
- Hematopoietic organoids: Opportunities and challenges in modeling human hematopoiesis and diseases in vitro.Stem cell reports · 2026Review
- Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow.bioRxiv : the preprint server for biology · 2025Article
- Article
- Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells.Stem cell reports · 2025Article
- Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells.STAR protocols · 2025Article
- Conversion of placental hemogenic endothelial cells to hematopoietic stem and progenitor cells.Cell discovery · 2025Article
- NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.Development (Cambridge, England) · 2024Article
- New insights into the endothelial origin of hematopoietic system inspired by "TIF" approaches.Blood science (Baltimore, Md.) · 2024Review
- Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors.Communications biology · 2024Article
- Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.Developmental cell · 2024Article
- The evolving hematopoietic niche during development.Frontiers in molecular biosciences · 2024Review
- The epicentre of haematopoiesis and osteogenesis.Nature cell biology · 2023Article
- Article
- Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium.Nature cell biology · 2023Article
- Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression.Nature communications · 2023Article
- Activation of lineage competence in hemogenic endothelium precedes the formation of hematopoietic stem cell heterogeneity.Cell research · 2023Article
- CD34Cell proliferation · 2023Article
66 more citing papers are in PubMed but not listed here.
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Authors and funding
18 authors at 7 institutions in 2 countries.
Raphael LisAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Charles C KarraschAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Michael G PoulosAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Balvir KunarAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
David RedmondInstitute for Computational Biomedicine &Institute for Precision Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Jose G Barcia DuranAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Chaitanya R BadweAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
William SchachterleAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Michael GinsbergAngiocrine Bioscience, San Diego, California 92130, USA.
Jenny XiangGenomics Resources Core Facility, Weill Cornell Medicine, New York, New York 10065, USA.
Arash Rafii TabriziStem Cell and Microenvironment Laboratory, Department of Obstetrics and Gynecology, Weill Cornell Medicine in Qatar, Education City, Qatar Foundation, PO box 24144, Doha, Qatar.
Koji ShidoAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Zev RosenwaksRonald O. Perelman and Claudia Cohen Center for Reproductive Medicine and Infertility, Weill Cornell Medicine, New York, New York 10065, USA.
Olivier ElementoInstitute for Computational Biomedicine &Institute for Precision Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Nancy A SpeckAbramson Family Cancer Research Institute, Institute for Regenerative Medicine and Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Jason M ButlerAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Joseph M ScanduraDepartment of Medicine, Hematology-Oncology, Weill Cornell Medicine and the New York Presbyterian Hospital, New York, New York 10065, USA.
Shahin RafiiAnsary Stem Cell Institute, Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York 10065, USA.
Cornell University · USWeill Cornell Medicine · USAngiocrine Bioscience (United States) · USCancer Research Institute · USFoundation for Reproductive Medicine · USPresbyterian Hospital · USWeill Cornell Medical College in Qatar · QA
Funding
Progenitor Cell Biology Consortium Administrative Coordinating CenterU01HL099997 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI TERRIN, MICHAEL L · 2009 to 2015
$29.9MBiochemistry of Leukemia Virus Core-Binding FactorR01HL091724 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI SPECK, NANCY · 2008 to 2024
$8.3MThe Proneural Perivascular Niche and Therapeutic ResponseU54CA163167 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI HOLLAND, ERIC C. · 2011 to 2015
$4.6MTraining Program in Developmental BiologyT32HD060600 · NICHD · WEILL MEDICAL COLL OF CORNELL UNIV · PI BAO, ZHIRONG, STUHLMANN, HEIDI · 2010 to 2024
$3.5MEvaluation of Pluripotent Stem Cell-Derived Blood Cells in Nonhuman Primate ModelR01HL115128 · NHLBI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI KIEM, HANS-PETER, RAFII, SHAHIN · 2013 to 2016
$3.3MDeciphering molecular determinants of vascular heterogeneity for organ repairR01HL128158 · NHLBI · WEILL MEDICAL COLL OF CORNELL UNIV · PI RAFII, SHAHIN · 2014 to 2018
$3.1MIdentification of vascular-derived signals for alveolar lung repairR01HL119872 · NHLBI · WEILL MEDICAL COLL OF CORNELL UNIV · PI RAFII, SHAHIN · 2013 to 2016
$2.3MIdentification of vascular inductive signals in liver regenerationR01DK095039 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI RAFII, SHAHIN · 2012 to 2016
$2.2MModulating signaling pathways in endothelial cells to abate leukemic progression.R01CA204308 · NCI · WEILL MEDICAL COLL OF CORNELL UNIV · PI BUTLER, JASON MATHEW · 2016 to 2020
$2.0MModulating endothelial-specific signaling to enhance functional hematopoiesis.R01HL133021 · NHLBI · WEILL MEDICAL COLL OF CORNELL UNIV · PI BUTLER, JASON MATHEW · 2016 to 2019
$1.7MContribution of the vascular niche to the hematopoietic reconstitution.R01HL097797 · NHLBI · WEILL MEDICAL COLL OF CORNELL UNIV · PI RAFII, SHAHIN · 2009 to 2012
$1.7MNCI NIH HHS R01 CA204308NCI NIH HHS U54 CA163167NHLBI NIH HHS R01 HL091724NHLBI NIH HHS R01 HL097797NHLBI NIH HHS R01 HL115128NHLBI NIH HHS R01 HL119872NHLBI NIH HHS R01 HL128158NHLBI NIH HHS R01 HL133021NHLBI NIH HHS U01 HL099997NICHD NIH HHS T32 HD060600NIDDK NIH HHS R01 DK095039NIH HHS HL099997NIH HHS HL115128NIH HHS HL119872NIH HHS HL128158NIH HHS HL133021NIH HHS NIH-R01 HL091724
Abstract
Developmental pathways that orchestrate the fleeting transition of endothelial cells into haematopoietic stem cells remain undefined. Here we demonstrate a tractable approach for fully reprogramming adult mouse endothelial cells to haematopoietic stem cells (rEC-HSCs) through transient expression of the transcription-factor-encoding genes Fosb, Gfi1, Runx1, and Spi1 (collectively denoted hereafter as FGRS) and vascular-niche-derived angiocrine factors. The induction phase (days 0-8) of conversion is initiated by expression of FGRS in mature endothelial cells, which results in endogenous Runx1 expression. During the specification phase (days 8-20), RUNX1
Indexed as
Cell DifferentiationCellular ReprogrammingAdaptive ImmunityAgingAnimalsCell LineCell LineageCell Self RenewalClone CellsCore Binding Factor Alpha 2 SubunitDNA-Binding ProteinsEndothelial CellsEndotheliumHematopoiesisHematopoietic Stem CellsHematopoietic Stem Cell TransplantationCore Binding Factor Alpha 2 SubunitDNA-Binding ProteinsFOSB protein, humanGFI1 protein, humanProto-Oncogene ProteinsProto-Oncogene Proteins c-fosProto-Oncogene Protein Spi-1RUNX1 protein, humanTrans-ActivatorsTranscription Factors
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.