ArticleNucleic acids research2017
Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- A passive protein environment offsets RNA folding energetics in cells.bioRxiv : the preprint server for biology · 2026Article
- Split RNA switch orchestrates pre- and post-translational control to enable cell type-specific gene expression.Nature communications · 2025Article
- AND-gated protease-activated nanosensors for programmable detection of anti-tumour immunity.Nature nanotechnology · 2025Article
- Advancing in vivo reprogramming with synthetic biology.Current opinion in biotechnology · 2024Review
- Rational design of microRNA-responsive switch for programmable translational control in mammalian cells.Nature communications · 2023Article
- Dual Input-Controlled Synthetic mRNA Circuit for Bidirectional Protein Expression Regulation.ACS synthetic biology · 2023Article
- Programmable mammalian translational modulators by CRISPR-associated proteins.Nature communications · 2023Article
- Synthetic circular RNA switches and circuits that control protein expression in mammalian cells.Nucleic acids research · 2023Article
- Sensing intracellular signatures with synthetic mRNAs.RNA biology · 2023Review
- Cytidine-containing tails robustly enhance and prolong protein production of synthetic mRNA in cell andMolecular therapy. Nucleic acids · 2022Article
- Engineering synthetic RNA devices for cell control.Nature reviews. Genetics · 2022Review
- A versatile and robust cell purification system with an RNA-only circuit composed of microRNA-responsive ON and OFF switches.Science advances · 2022Article
- Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells.Life (Basel, Switzerland) · 2021Review
- Chimeric RNA-binding protein-based killing switch targeting hepatocellular carcinoma cells.Molecular therapy. Nucleic acids · 2021Article
- Developments of Riboswitches and Toehold Switches for Molecular Detection-Biosensing and Molecular Diagnostics.International journal of molecular sciences · 2020Review
- N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells.Nucleic acids research · 2020Article
- Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery.Nature communications · 2020Article
- Nucleotide Modification Alters MicroRNA-Dependent Silencing of MicroRNA Switches.Molecular therapy. Nucleic acids · 2019Article
- Synthetic RNA-based logic computation in mammalian cells.Nature communications · 2018Article
- Synthetic switch-based baculovirus for transgene expression control and selective killing of hepatocellular carcinoma cells.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology has great potential for future therapeutic applications including autonomous cell programming through the detection of protein signals and the production of desired outputs. Synthetic RNA devices are promising for this purpose. However, the number of available devices is limited due to the difficulty in the detection of endogenous proteins within a cell. Here, we show a strategy to construct synthetic mRNA devices that detect endogenous proteins in living cells, control translation and distinguish cell types. We engineered protein-binding aptamers that have increased stability in the secondary structures of their active conformation. The designed devices can efficiently respond to target proteins including human LIN28A and U1A proteins, while the original aptamers failed to do so. Moreover, mRNA delivery of an LIN28A-responsive device into human induced pluripotent stem cells (hiPSCs) revealed that we can distinguish living hiPSCs and differentiated cells by quantifying endogenous LIN28A protein expression level. Thus, our endogenous protein-driven RNA devices determine live-cell states and program mammalian cells based on intracellular protein information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.