ArticlePloS one2017
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- ThemBio · 2026Article
- Review
- Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells.Medical microbiology and immunology · 2024Article
- The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole.Infection and immunity · 2023Article
- Molecular pathogenesis ofFrontiers in cellular and infection microbiology · 2023Review
- Genome organization and genomics inFrontiers in cellular and infection microbiology · 2023Review
- CT295 IsFrontiers in cellular and infection microbiology · 2022Article
- The Type III Secretion Effector CteG Mediates Host Cell Lytic Exit ofFrontiers in cellular and infection microbiology · 2022Article
- The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells.PloS one · 2022Article
- Article
- Got mutants? How advances in chlamydial genetics have furthered the study of effector proteins.Pathogens and disease · 2021Review
- Article
- The Putative Type III SecretedFrontiers in microbiology · 2020Article
- The multiple functions of the numerousMicrobial cell (Graz, Austria) · 2019Review
- CteG is a Chlamydia trachomatis effector protein that associates with the Golgi complex of infected host cells.Scientific reports · 2019Article
- Ptr/CTL0175 Is Required for the Efficient Recovery ofFrontiers in microbiology · 2019Article
- Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to InvasivemBio · 2018Article
- The Human Centrosomal Protein CCDC146 BindsFrontiers in cellular and infection microbiology · 2018Article
- Transformation of Chlamydia: current approaches and impact on our understanding of chlamydial infection biology.Microbes and infectionReview
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital infections. It multiplies exclusively within an intracellular membrane-bound vacuole, the inclusion, and uses a type III secretion system to manipulate host cells by injecting them with bacterially-encoded effector proteins. In this work, we characterized the expression and subcellular localization in infected host cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously showed to be type III secretion substrates. Transcriptional analyses in C. trachomatis confirmed the prediction that ct142, ct143 and ct144 are organized in an operon and revealed that their expression is likely driven by the main σ factor, σ66. In host cells infected by C. trachomatis, production of CT142 and CT143 could be detected by immunoblotting from 20-26 h post-infection. Immunofluorescence microscopy of infected cells revealed that from 20 h post-infection CT143 appeared mostly as globular structures outside of the bacterial cells but within the lumen of the inclusion. Furthermore, immunofluorescence microscopy of cells infected by C. trachomatis strains carrying plasmids producing CT142, CT143, or CT144 under the control of the ct142 promoter and with a C-terminal double hemagglutinin (2HA) epitope tag revealed that CT142-2HA, CT143-2HA or CT144-2HA showed an identical localization to chromosomally-encoded CT143. Moreover, CT142-2HA or CT144-2HA and CT143 produced by the same bacteria co-localized in the lumen of the inclusion. Overall, these data suggest that the CT142, CT143, and CT144 type III secretion substrates are secreted into the lumen of the inclusion where they might form a protein complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.