Evidence map›Paper›PMID 28672397›Full record

ReviewInvestigative ophthalmology & visual science2017

Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging.

Andrew W Browne, Cosimo Arnesano, Narine Harutyunyan, Thien Khuu, Juan Carlos Martinez, Harvey A Pollack, David S Koos, Thomas C Lee, Scott E Fraser, Rex A Moats and 2 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Hyperspectral Imaging and the Retina: Worth the Wave?Translational vision science & technology · 2020
    Pooled it
  2. Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Andrew W BrowneUSC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California, United States.
Cosimo ArnesanoTranslational Imaging Center, University of Southern California, Los Angeles, California, United States 3Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States.
Narine HarutyunyanThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States.
Thien KhuuThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States.
Juan Carlos MartinezUSC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California, United States.
Harvey A PollackThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States 6Department of Radiology, Children's Hospital Los Angeles, Los Angeles, California, United States.
David S KoosThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States 6Department of Radiology, Children's Hospital Los Angeles, Los Angeles, California, United States.
Thomas C LeeUSC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California, United States 4The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States.
Scott E FraserTranslational Imaging Center, University of Southern California, Los Angeles, California, United States 3Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States 5The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States 7Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States.
Rex A MoatsThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States 6Department of Radiology, Children's Hospital Los Angeles, Los Angeles, California, United States 7Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States.
Jennifer G AparicioThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States.
David CobrinikUSC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California, United States 4The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States 5The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States 8Department of Biochemistry & Molecular Medicine, and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, Los Angeles, California, United States.

Funding

Human Specific Signaling Circuitry in Cone Precursor DevelopmentR01EY026661 · NEI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI COBRINIK, DAVID · 2016 to 2019
$1.7M
Modeling Optic Nerve Hypoplasia with Patient iPSC-Derived Retinal Ganglion CellsR21EY025419 · NEI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI BORCHERT, MARK STEPHEN, COBRINIK, DAVID · 2015 to 2016
$454k
NEI NIH HHS R01 EY026661NEI NIH HHS R21 EY025419
6 · The paper itself

Abstract

Purpose: Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for investigating retinal development, pathophysiology, and cellular therapies. In contrast to histologic analysis in which multiple specimens fixed at different times are used to reconstruct developmental processes, repeated analysis of the same living organoids provides a more direct means to characterize changes. New live imaging modalities can provide insights into retinal organoid structure and metabolic function during in vitro growth. This study employed live tissue imaging to characterize retinal organoid development, including metabolic changes accompanying photoreceptor differentiation. Methods: Live hPSC-derived retinal organoids at different developmental stages were examined for microanatomic organization and metabolic function by phase contrast microscopy, optical coherence tomography (OCT), fluorescence lifetime imaging microscopy (FLIM), and hyperspectral imaging (HSpec). Features were compared to those revealed by histologic staining, immunostaining, and microcomputed tomography (micro-CT) of fixed organoid tissue. Results: We used FLIM and HSpec to detect changes in metabolic activity as organoids differentiated into organized lamellae. FLIM detected increased glycolytic activity and HSpec detected retinol and retinoic acid accumulation in the organoid outer layer, coinciding with photoreceptor genesis. OCT enabled imaging of lamellae formed during organoid maturation. Micro-CT revealed three-dimensional structure, but failed to detect lamellae. Conclusions: Live imaging modalities facilitate real-time and nondestructive imaging of retinal organoids as they organize into lamellar structures. FLIM and HSpec enable rapid detection of lamellar structure and photoreceptor metabolism. Live imaging techniques may aid in the continuous evaluation of retinal organoid development in diverse experimental and cell therapy settings.

Indexed as

Diagnostic Techniques, OphthalmologicalHumansMicroscopy, FluorescenceOrganoidsPluripotent Stem CellsRetinaTomography, Optical CoherenceX-Ray Microtomography

Identifiers

PMID28672397
PMCPMC5495152

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.