ArticleAngewandte Chemie (International ed. in English)2017
Long-Term Live-Cell STED Nanoscopy of Primary and Cultured Cells with the Plasma Membrane HIDE Probe DiI-SiR.
Article in Angewandte Chemie (International ed. in English), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Expanding the multiplexing capability of HIDE probes via fluorescence lifetime imaging microscopy.Methods in enzymology · 2026Article
- Fluorescent Solvatochromic Probes for Long-Term Imaging of Lipid Order in Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Long-term super-resolution inner mitochondrial membrane imaging with a lipid probe.Nature chemical biology · 2024Article
- Chemical Approaches for Measuring and Manipulating Lipids at the Organelle Level.Cold Spring Harbor perspectives in biology · 2023Review
- Molecular Engineering of Near-Infrared Fluorescent Probes for Cell Membrane Imaging.Molecules (Basel, Switzerland) · 2023Review
- Water-soluble extracellular vesicle probes based on conjugated oligoelectrolytes.Science advances · 2023Article
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Imaging organelle membranes in live cells at the nanoscale with lipid-based fluorescent probes.Current opinion in chemical biology · 2021Review
- Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons.RSC chemical biology · 2021Article
- DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.Nano letters · 2020Article
- Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.Journal of physics D: Applied physics · 2020Review
- Espin distribution as revealed by super-resolution microscopy of stereocilia.American journal of translational research · 2020Article
- A new approach to silicon rhodamines by Suzuki-Miyaura coupling - scope and limitations.Beilstein journal of organic chemistry · 2019Article
- Synthesis of a dihalogenated pyridinyl silicon rhodamine for mitochondrial imaging by a halogen dance rearrangement.Beilstein journal of organic chemistry · 2019Article
- Greasing the Wheels of Lipid Biology with Chemical Tools.Trends in biochemical sciences · 2018Review
- Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy.Nature biotechnology · 2018Article
- HIDE Probes: A New Toolkit for Visualizing Organelle Dynamics, Longer and at Super-Resolution.Biochemistry · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Super-resolution imaging of live cells over extended time periods with high temporal resolution requires high-density labeling and extraordinary fluorophore photostability. Herein, we achieve this goal by combining the attributes of the high-density plasma membrane probe DiI-TCO and the photostable STED dye SiR-Tz. These components undergo rapid tetrazine ligation within the plasma membrane to generate the HIDE probe DiI-SiR. Using DiI-SiR, we visualized filopodia dynamics in HeLa cells over 25 min at 0.5 s temporal resolution, and visualized dynamic contact-mediated repulsion events in primary mouse hippocampal neurons over 9 min at 2 s temporal resolution. HIDE probes such as DiI-SiR are non-toxic and do not require transfection, and their apparent photostability significantly improves the ability to monitor dynamic processes in live cells at super-resolution over biologically relevant timescales.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.