Evidence map›Paper›PMID 28717196›Full record

ArticleScientific reports2017

TRIB1 is a positive regulator of hepatocyte nuclear factor 4-alpha.

Sébastien Soubeyrand, Amy Martinuk, Ruth McPherson

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 3 pooled it
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 3 syntheses or guidelines pooled it, 29 citations in OpenAlex.

  1. Pooled it
  2. Effect ofCardiovascular therapeutics · 2023
    Pooled it
  3. Pooled it
  4. Article
  5. Article
  6. Genes · 2024
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Frontiers in genetics · 2020
    Article
  16. Article
  17. Trouble With Tribbles-1.Arteriosclerosis, thrombosis, and vascular biology · 2019
    Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sébastien SoubeyrandAtherogenomics Laboratory, University of Ottawa Heart Institute, Ottawa, Canada. ssoubeyrand@ottawaheart.ca.ORCID 0000-0002-9317-301X
Amy MartinukAtherogenomics Laboratory, University of Ottawa Heart Institute, Ottawa, Canada.
Ruth McPhersonAtherogenomics Laboratory, University of Ottawa Heart Institute, Ottawa, Canada. rmcpherson@ottawaheart.ca.
University of Ottawa · CA

Funding

CIHR
6 · The paper itself

Abstract

The TRIB1 locus has been linked to both cardiovascular disease and hepatic steatosis. Recent efforts have revealed TRIB1 to be a major regulator of liver function, largely, but not exclusively, via CEBPA degradation. We recently uncovered a functional interaction between TRIB1 and HNF4A, another key regulator of hepatic function, whose molecular underpinnings remained to be clarified. Here we have extended these findings. In hepatoma models, HNF4A levels were found to depend on TRIB1, independently of its impact on CEBPA. Using a reporter assay model, MTTP reporter activity, which depends on HNF4A, positively correlated with TRIB1 levels. Confocal microscopy demonstrated partial colocalization of TRIB1 and HNF4A. Using overexpressed proteins we demonstrate that TRIB1 and HNF4A can form complexes in vivo. Mapping of the interaction interfaces identified two distinct regions within TRIB1 which associated with the N-terminal region of HNF4A. Lastly, the TRIB1-HNF4A interaction resisted competition with a CEPBA-derived peptide, suggesting different binding modalities. Together these findings establish that TRIB1 is required for HNF4A function. This regulatory axis represents a novel CEBPA-independent aspect of TRIB1 function predicted to play an important role in liver physiology.

Indexed as

Binding SitesCarcinoma, HepatocellularCell Line, TumorGene Expression Regulation, NeoplasticHEK293 CellsHepatocyte Nuclear Factor 4Hep G2 CellsHumansIntracellular Signaling Peptides and ProteinsLiver NeoplasmsProtein BindingProtein Serine-Threonine KinasesHepatocyte Nuclear Factor 4HNF4A protein, humanIntracellular Signaling Peptides and ProteinsProtein Serine-Threonine KinasesTRIB1 protein, human

Identifiers

PMID28717196
PMCPMC5514136
OpenAlexW2734972233

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.