Evidence map›Paper›PMID 28779686›Full record

ArticleVirology2017

Deep sequencing of RSV from an adult challenge study and from naturally infected infants reveals heterogeneous diversification dynamics.

Jessica W Lau, Young-In Kim, Ryan Murphy, Ruchi Newman, Xiao Yang, Michael Zody, John DeVincenzo, Yonatan H Grad

Open access · hybridAbstract read
In one paragraph

Article in Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jessica W LauDepartment of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA 02115, United States.
Young-In KimDepartment of Pediatrics, University of Tennessee School of Medicine, Memphis, TN 38103, United States; Children's Foundation Research Institute at LeBonheur Children's Hospital, Memphis, TN 38103, United States.
Ryan MurphyDepartment of Pediatrics, University of Tennessee School of Medicine, Memphis, TN 38103, United States.
Ruchi NewmanBroad Institute of Harvard and MIT, Cambridge, MA 02142, United States.
Xiao YangBroad Institute of Harvard and MIT, Cambridge, MA 02142, United States.
Michael ZodyBroad Institute of Harvard and MIT, Cambridge, MA 02142, United States.
John DeVincenzoDepartment of Pediatrics, University of Tennessee School of Medicine, Memphis, TN 38103, United States; Children's Foundation Research Institute at LeBonheur Children's Hospital, Memphis, TN 38103, United States; Department of Microbiology, Immunology, and Biochemistry, University of Tennessee School of Medicine, Memphis, TN 38103, United States.
Yonatan H GradDepartment of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA 02115, United States; Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, United States. Electronic address: ygrad@hsph.harvard.edu.
Broad Institute · USUniversity of Tennessee Health Science Center · USHarvard University · US

Funding

Genomic epidemiology of Neisseria gonorrhoeae with elevated MICs to cefiximeK08AI104767 · NIAID · HARVARD SCHOOL OF PUBLIC HEALTH · PI GRAD, YONATAN H · 2013 to 2015
$547k
NIAID NIH HHS HHSN272200900018CNIAID NIH HHS K08 AI104767
6 · The paper itself

Abstract

As RNA virus mutation occurs during replication within host cells, we hypothesized that viral evolution during acute infections in healthy hosts reflects host immune pressure. We therefore investigated the within-host diversification of human respiratory syncytial virus (RSV), a highly prevalent cause of acute respiratory infections. We evaluated healthy adults experimentally infected with an identical inoculum and infants hospitalized with naturally acquired infections. In aggregate, viral diversification in adults peaked at day 3, with overrepresentation of diversity in the matrix protein 2 (M2) and non-structural protein 2 (NS2) genes. In one subject, delayed viral clearance was accompanied by a late peak of diversity at day 10 in known and predicted B and T cell epitopes. In contrast, infant infections showed much less viral diversity. Our findings suggest multiple overlapping mechanisms for early control of acute viral infections, which may differ between age groups and host immune responses.

Indexed as

Genetic VariationAdultEpitopes, B-LymphocyteEpitopes, T-LymphocyteEvolution, MolecularHigh-Throughput Nucleotide SequencingHumansInfantMutationRespiratory Syncytial Virus, HumanRespiratory Syncytial Virus InfectionsViral ProteinsEpitopes, B-LymphocyteEpitopes, T-LymphocyteViral ProteinsDeep sequencingGenomicsRSVViral evolutionWithin-host diversity

Identifiers

PMID28779686
PMCPMC5580249
OpenAlexW2745054205

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.