Evidence map›Paper›PMID 28782252›Full record

ArticleBJU international2017

cAMP-dependent post-translational modification of neuronal nitric oxide synthase neuroprotects penile erection in rats.

Serkan Karakus, Biljana Musicki, Justin D La Favor, Arthur L Burnett

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BJU international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. A tissue bandage for pelvic ganglia injury.Nature communications · 2024
    Article
  6. Review
  7. Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Serkan KarakusThe James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Biljana MusickiThe James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Justin D La FavorThe James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Arthur L BurnettThe James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Johns Hopkins University · US

Funding

NOS Regulation in the PenisR01DK067223 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI BURNETT, ARTHUR LOUIS · 2005 to 2015
$3.5M
Regulation of eNOS by S-Nitrosylation in Diet Associated Erectile DysfunctionF32DK100082 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI LAFAVOR, JUSTIN DAVID · 2014 to 2016
$149k
NIDDK NIH HHS F32 DK100082NIDDK NIH HHS R01 DK067223
6 · The paper itself

Abstract

objectivesTo evaluate neuronal nitric oxide (NO) synthase (nNOS) phosphorylation, nNOS uncoupling, and oxidative stress in the penis and major pelvic ganglia (MPG), before and after the administration of the cAMP-dependent protein kinase A (PKA) agonist colforsin in a rat model of bilateral cavernous nerve injury (BCNI),which mimics nerve injury after prostatectomy. MATERIALS AND

methodsAdult male Sprague-Dawley rats were divided into BCNI and sham-operated groups. Each group included two subgroups: vehicle and colforsin (0.1 mg/kg/day i.p.). After 3 days, erectile function (intracavernosal pressure) was measured and penis and MPG were collected for molecular analyses of phospho (P)-nNOS (Ser-1412 and Ser-847), total nNOS, nNOS uncoupling, binding of protein inhibitor of nNOS (PIN) to nNOS, gp91

resultsErectile function was decreased 3 days after BCNI and normalized by colforsin. nNOS phosphorylation on both positive (Ser-1412) and negative (Ser-847) regulatory sites, and nNOS uncoupling, were increased after BCNI in the penis and MPG, and normalized by colforsin. H

conclusionCollectively, BCNI impairs nNOS function in the penis and MPG by mechanisms involving its phosphorylation and uncoupling in association with increased oxidative stress, resulting in erectile dysfunction. PKA activation by colforsin reverses these molecular changes and preserves penile erection in the face of BCNI.

Indexed as

Neuroprotective AgentsNitric Oxide Synthase Type IProtein Processing, Post-TranslationalAnimalsBlood PressureBody WeightErectile DysfunctionGangliaMaleOxidative StressPelvisPenile ErectionPhosphorylationRatsRats, Sprague-DawleyNeuroprotective AgentsNitric Oxide Synthase Type Icavernous nerve injurycolforsinneuropathic erectile dysfunctionnNOS uncouplingoxidative stressphospho-nNOS (Ser-1412 and Ser-847)

Identifiers

PMID28782252
PMCPMC5718212
OpenAlexW2744047754

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.