ArticleBMC genomics2017
Characterization of the peripheral blood transcriptome and adaptive evolution of the MHC I and TLR gene families in the wolf (Canis lupus).
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Pooled it
- Evolutionary Response Patterns of the RodentEcology and evolution · 2026Article
- Signatures of Radiation-Induced Stress and Putative Selection on Immune Targets in Chornobyl Wolves.Molecular ecology · 2026Article
- Evolutionary Diversification and Adaptive Evolution Analysis of the Plant HD-Zip IV Subfamily.Genes · 2025Article
- Potential Adaptive Introgression From Dogs in Iberian Grey Wolves (Canis lupus).Molecular ecology · 2025Article
- Dog Domestication Strongly Relied on Translation Regulation According to Differential Gene Expression Analysis.Animals : an open access journal from MDPI · 2024Article
- Twelve toll-like receptor (TLR) genes in the family Equidae - comparative genomics, selection and evolution.Veterinary research communications · 2024Article
- Article
- Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.Immunogenetics · 2022Article
- Transcriptome analysis provides new insights into cold adaptation of corsac fox (Ecology and evolution · 2022Article
- Species and population specific gene expression in blood transcriptomes of marine turtles.BMC genomics · 2021Article
- Article
- Evolution of major histocompatibility complex class I genes in the sableEcology and evolution · 2020Article
- Evolutionary History of the Toll-Like Receptor Gene Family across Vertebrates.Genome biology and evolution · 2020Article
- Analysis of substitution rates showed that TLR5 is evolving at different rates among mammalian groups.BMC evolutionary biology · 2019Article
- Origin and evolution of the major histocompatibility complex class I region in eutherian mammals.Ecology and evolution · 2019Article
- Evolution of MHC class I genes in Eurasian badgers, genus Meles (Carnivora, Mustelidae).Heredity · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, because it is well adapted to various ecological niches and their corresponding pathogen environments. Immunological competence is a crucial factor involved in adapting to a changing environment and fighting pathogen infection in animals. In this study, the peripheral blood transcriptome of wolves was generated via RNA-seq to advance understanding of the wolf immunome, with a special focus on the major histocompatibility complex class I (MHC I) and toll-like receptor (TLR) gene families, which are involved in pathogen recognition and defense.
resultsThe blood transcriptomic libraries of eight wolves originating from Tibet and Inner Mongolia were sequenced, and approximately 383 million reads were generated. Using a genome-guided assembly strategy, we obtained 123,851 unigenes, with a mean length of 845 bp and an N50 length of 1121 bp. On the basis of BLAST searches against the NCBI non-redundant protein database (Nr), a total of 36,192 (29.22%) unigenes were annotated. For functional classification, 24,663 unigenes were assigned to 13,016 Gene Ontology (GO) terms belonging to 51 sub-categories of the three main GO categories. Additionally, 7682 unigenes were classified into 6 Kyoto Encyclopedia of Genes and Genomes (KEGG) categories, in which the most represented functional sub-categories were signal transduction and the immune system, and 16,238 unigenes were functionally classified into 25 Eukaryotic Orthologous Groups (KOG) categories. We observed an overall higher ω (d
conclusionsThis study represents the first attempt to characterize the blood transcriptome of the wolf and to highlight the value of investigating the immune system. Balancing selection and recombination have contributed to the historical evolution of wolf MHC I genes. Moreover, TLRs in carnivores have undergone adaptive evolution against the background of purifying selection, and a high level of adaptive evolution was detected in the wolf TLR system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.