Evidence map›Paper›PMID 28792749›Full record

ArticleBiochemistry2017

HIDE Probes: A New Toolkit for Visualizing Organelle Dynamics, Longer and at Super-Resolution.

Alexander D Thompson, Joerg Bewersdorf, Derek Toomre, Alanna Schepartz

Abstract read
In one paragraph

Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Lipid Expansion Microscopy.Journal of the American Chemical Society · 2022
    Article
  11. Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Review
  12. Article
  13. Review
  14. Article
  15. A real-time, click chemistry imaging approach reveals stimulus-specific subcellular locations of phospholipase D activity.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  16. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alexander D ThompsonDepartment of Chemistry, ‡Department of Molecular, Cellular and Developmental Biology, §Department of Cell Biology, and ∥Department of Biomedical Engineering, Yale University , New Haven, Connecticut 06520-8107, United States.
Joerg BewersdorfDepartment of Chemistry, ‡Department of Molecular, Cellular and Developmental Biology, §Department of Cell Biology, and ∥Department of Biomedical Engineering, Yale University , New Haven, Connecticut 06520-8107, United States.
Derek ToomreDepartment of Chemistry, ‡Department of Molecular, Cellular and Developmental Biology, §Department of Cell Biology, and ∥Department of Biomedical Engineering, Yale University , New Haven, Connecticut 06520-8107, United States.
Alanna SchepartzDepartment of Chemistry, ‡Department of Molecular, Cellular and Developmental Biology, §Department of Cell Biology, and ∥Department of Biomedical Engineering, Yale University , New Haven, Connecticut 06520-8107, United States.ORCID 0000-0003-2127-3932

Funding

Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI GERALD I SHULMAN · 1993 to 2026
$44.0M
Enabling Nanoscale Dynamic Imaging of Vesicles and OrganellesR01GM118486 · NIGMS · YALE UNIVERSITY · PI BADDELEY, DAVID, BEWERSDORF, JOERG · 2016 to 2019
$3.4M
Surveying protein partnerships and assembly with bipartite tetracysteine displayR01GM083257 · NIGMS · YALE UNIVERSITY · PI SCHEPARTZ, ALANNA · 2008 to 2018
$3.2M
Expanding the HIDE nanoscopy toolbox: More organelles, colors, and modalitiesR01GM131372 · NIGMS · YALE UNIVERSITY · PI SCHEPARTZ, ALANNA · 2019 to 2019
$299k
Tailored Probes for Super-Resolution MicroscopyF31GM119259 · NIGMS · YALE UNIVERSITY · PI THOMPSON, ALEXANDER · 2016 to 2017
$66k
NIDDK NIH HHS P30 DK045735NIGMS NIH HHS F31 GM119259NIGMS NIH HHS R01 GM083257NIGMS NIH HHS R01 GM118486NIGMS NIH HHS R01 GM131372Wellcome Trust 095927/A/11/ZWellcome Trust 203285/Z/16/Z
6 · The paper itself

Abstract

Living cells are complex and dynamic assemblies that carefully sequester and orchestrate multiple diverse processes that enable growth, division, regulation, movement, and communication. Membrane-bound organelles such as the endoplasmic reticulum, mitochondria, plasma membrane, and others are integral to these processes, and their functions demand dynamic reorganization in both space and time. Visualizing these dynamics in live cells over long time periods demands probes that label discrete organelles specifically, at high density, and withstand long-term irradiation. Here we describe the evolution of our work on the development of a set of high-density environmentally sensitive (HIDE) membrane probes that enable long-term, live-cell nanoscopy of the dynamics of multiple organelles in live cells using single-molecule switching and stimulated emission depletion imaging modalities.

Indexed as

HeLa CellsHumansIntracellular MembranesMolecular ImagingOrganellesSignal-To-Noise RatioTime Factors

Identifiers

PMID28792749
PMCPMC5854879

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.